LabAutomation 2006 - SLAS

TP51
Thomas Rothmann
Qiagen Gmbh
Hilden, Germany
thomas.rothmann@qiagen.com
Where Laboratory Technologies Emerge and Merge
Co-Author(s)
Thorsten Voss
Ralf Wyrich
Daniel Langendörfer
PreAnalytiX
Uwe Oelmüller
Lynne Rainen
PreAnalytiX
High-Throughput Automation for RNA Isolation From Blood Stabilized in PAXgene
Blood RNA Tubes Allows State-of-the-Art Gene Expression Profiling
Artificial modifications of the RNA content and profile blood samples post-phlebotomy caused by degradation and gene induction is well
documented. Doubtful or inconsistent results are the consequence, especially for quantitative or semi-quantitative analytical methods such
as qRT-PCR assays and microarrays. There is a need, therefore, for stabilization of cellular RNA species to freeze the gene expression
profile at the time of blood collection. The manual version of the PAXgene Blood RNA System is commonly used to address this problem.
To allow a higher sample throughput combined with a controlled and completely documented process (e.g. for clinical trials), we have
designed protocols and kits for two state-of-the-art robotic platforms. This study was conducted to show the performance of these robotic
solutions. The prepared RNA was tested in several qRT-PCR assays and on Affymetrix GeneChips. Results comparing the RNA quality
from manual versus automated protocols on the BioRobot ® MDx and the BioRobot® Universal System (QIAGEN) will be presented.
TP52
Jas Sanghera
TTP LabTech
Melbourn, Hertfordshire, United Kingdom
jas.sanghera@ttplabtech.com
Co-Author(s)
Richard Shaw, TTP LabTech
Simon Tullet, TTP LabTech
Joby Jenkins, TTP LabTech
A Flexible Solution for High-Speed Sample Cherry-Picking From Frozen Storage
To successfully automate sample storage and retrieval, compound samples must be quickly and reliably delivered to the location where
they are required. The implementation of the store itself should not cause a company major upheaval and reorganisation, and – especially
important for smaller companies – the store must be able to grow with a company’s business. The comPOUND sample store (TTP LabTech)
is a modular and flexible system that can be installed almost anywhere and offers a range of solutions from manual, front of store retrieval,
through remote delivery, walk away overnight processing to full store-to-assay-plate automation with no operator intervention. This fully
scaleable approach to compound storage also allows sample throughputs to increase with the library size to match process needs.
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