LabAutomation 2006 - SLAS

TP45
Nick Price
Invitrogen Corporation
Carlsbad, California
nick.price@invitrogen.com
LabAutomation2006
Co-Author(s)
Byung-in Lee
Lansha Peng
Karl H. Hecker
PureLink 96 Purification Kits: High-throughput isolation of nucleic acid suitable for all
downstream applications
The ever increasing demand for high-throughput applications for nucleic acid purification have led to development of a number of
automated protocols. Here we describe the use of the Tecan Freedom EVO ® liquid handling platform equipped with a vacuum separation
system, to automate purification of plasmid DNA, PCR products, and total RNA using Invitrogen’s PureLink 96-well purification kits.
The PureLink 96 HQ Plasmid DNA Purification Kit is based on a modified alkaline cell lysis procedure and selective plasmid DNA
binding to glass microfiber filters. Eluted plasmid DNA can be used directly in all relevant downstream applications. The PureLink 96
Total RNA Purification Kit is based on binding of total RNA from crude lysate to silica-based membranes. The lysate can be prepared
from bacteria, yeast, mammalian and plant cells and tissue. The eluted total RNA is of high quality, as demonstrated by the absence
of genomic DNA contamination and reliable qRT-PCR performance. When a PCR product is used in downstream applications such as
cloning or sequencing it is often necessary to purify the DNA fragment from reaction components. The PureLink 96 PCR Purification
Kit provides fast and quantitative recovery of PCR products. The purified DNA can be used in all relevant downstream applications. The
above-mentioned kits for nucleic acid purification provide turnkey, completely hands-free nucleic acid purification solutions. The scripts give
the added flexibility of processing from 1 up to 96 samples. The protocols are fast and reliable producing high quality nucleic acid products
suitable for all relevant downstream applications.
TP46
Giovanna Prout
Aurora Discovery, Inc.
San Diego, California
giovanna_prout@auroradiscovery.com
Using Aurora Discovery’s BioRAPTR FRD for Improved Performance of
High-Throughput Bead-based Assays
Bead-based assays are a cost effective alternative to a number of currently-utilized biological assays. New applications for bead-based
assays have surfaced in the biotech, pharmaceutical and genomic fields, but have been limited to 96-well and 384-well format. These
applications range from biodefense and forensics to immunoassays and genotyping. With industry rapidly progressing towards high
throughput automation of these bead-based applications, the need for precision low-volume liquid dispensers has never been greater.
Yet, despite this progression, many difficulties arise when dispensing low volumes of suspended material that have not been addressed by
most low-volume liquid dispensers. These challenges include non-uniform distribution of the particles across the microplates, tips clogging,
parameter restrictions including pressure and speed adjustment, and limitations of dispensing into designated areas within a plate. Aurora
Discovery’s BioRAPTR Flying Reagent Dispenser (BioRAPTR FRD) offers a novel solution for precision high throughput dispensing of
beads into 384-well, 1536-well, and 3456-well microplates using efficient, non-contact micro-solenoid valve dispensing starting at 100nL
dispense volumes. This poster will describe how the BioRAPTR FRD addresses the challenges of dispensing bead-based solutions and
explores the experimental results of dispensing a variety of bead sizes, densities, and volumes in 1536-well microplates.
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