LabAutomation 2006 - SLAS
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LabAutomation 2006 - SLAS

LabAutomation 2006 - SLAS LabAutomation 2006 - SLAS

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TP29 Hanh Le PerkinElmer Life and Analytical Sciences Boston, Massachusetts hanh.le@perkinelmer.com LabAutomation2006 Co-Author(s) Harry Harney Stephen Hurt Robert Stanaker PerkinElmer Life and Analytical Sciences Rajesh Manchanda Utilization of the ATPlite 1step Detection System for Homogenous Automated Cytotoxicity Assays A number of stategies are currently being implemented in the effort to increase the success rate in identifying new potential therapeutic lead compounds, and reducing the number of late stage failures in clinical trials. One approach is to perform ADME/Tox profiling at an earlier stage in the drug discovery process, leading therefore to the need for increased efficiency in carrying out these asssays. ATPlite 1step is a homogenous, luciferase-based luminescence assay system for the quantitative measurement of proliferation and cytotoxicity in cultured mammalian cells. The assay is based on the measurement of ATP as a marker for cell viability, and is both highly sensitive and easy-to-use. The single addition format for the assay makes it particulary suitable for automation. We have used the ATPlite 1step system to perform automated cytoxicity assays on the Cellular Workstation, an integrated walk-way system for cellular assays. The components of the workstation as configured for this assay include an Evolution P3 for reagent dispensing, EnVision microplate reader,CataLyst Express robotic arm, Cytomat microplate incubator and POLARATM scheduling sorftware. Data will be presented demonstrating the assay protocol and the automation methodology. TP30 Anthony Lemmo Entevis Inc Sudbury, Massachusetts tlemmo@entevis.com Automated Dispensing of Solid Powders and Viscous Reagents: Enabling Solutions for Material Discovery, Development and Optimization Two major challenges in the automation of aspects of new materials development are the dispensing of solid powders and dispensing high viscosity reagents. We have developed automated, benchtop systems to address both of these key challenges. The solid dispensing system is capable of dispensing powders with bulk densities ranging from 0.03 to 3 in the mass range from 100 micrograms to hundreds of milligrams with %CV’s of 15% or better. The viscous fluid dispensing system can handle reagents with viscosities that range from 0.01 to 900 cp and cover the volume range from 1 microliter to hundreds of milliliters with %CV’s typically < 2%. These systems, either used as stand-alone solutions, or in a combined workstation, have utility in the pharmaceutical development process for the automation of aqueous solubility, salt selection, polymorph screening and animal dosing experiments. They can also have a major impact in compound management operation, where the majority of samples are either solid powders or “difficult” liquids (e.g. oils, waxes, etc.). 166

TP31 Sophia Liang Aurora Biomed Vancouver, Canada sophia@aurorabiomed.com Where Laboratory Technologies Emerge and Merge Co-Author(s) David Wicks Joy Goswami Dong Liang, Aurora Biomed Inc. Optimization of A Robotic System For Automation Of Peptide Array Printing Printing high-density arrays have become an important tool in drug screening, molecular biology, and genetic analysis. Printing multiple samples onto a solid substrate allows researchers to efficiently screen thousands of conditions in a very small space, thereby saving time and money. In order to screen for peptides that inhibit bacterial growth, Aurora Biomed Inc. performed array printing onto a solid substrate using variations of a peptide sequence with its versatile VERSA1000 liquid handling workstation. In order to optimize the nano-array technology and print volumes, careful standardization procedures were carried out on automation parameters such as pulse length, pressure, solvent concentration, solvent composition, substrate surface treatment, robotic movement and speed. Results from our experiments conclusively showed that the optimization was successful in generating high-density arrays for antibodies and peptides at volumes as low as 15nL on the epoxy coated glass slides. We present here, the optimization procedure of our present printing technology and explore the applications of the VERSA1000 in array printing. TP32 David Lorenz Deerac Fluidics Dublin 2, Ireland david.lorenz@deerac.com Co-Author(s) Aoife Gallagher, Deerac Fluidics Brad Larson Tracy Worzella Michael Bjerke Promega Corporation Eric Matthews, BMG Labtech High-Throughput Automation of a Dual Reporter Assay in Low-Volume 384 & 1536- Well Plate Formats Using the Deerac Fluidics’ EquatorTM HTS- Eight Tip Pipetting System, Promega’s Chroma-Luc Technology, and BMG LABTECH’s PHERAstar Microplate Reader The drive towards miniaturization within the pharmaceutical and biotechnology fields has created a need for liquid handling technologies that accurately deliver low volume reagents to high-density plates. This has also created a need for simple, fully scaleable assays in low volumes. Here we demonstrate the successful combination of both through the use of the Equator HTS - Eight Tip Pipetting System, and the dual color Chroma-Luc technology. Cellular lysates, containing the green CBG99luc and red CBRluc genes, followed by Chroma-Glo reagent, were dispensed in low-volume 384, and 1536-well formats using volumes ranging from 10ul to 500nl. Luminescence from the two luciferases was then simultaneously measured using the BMG LABTECH PHERAstar plate reader. The exceptional Z’ Factor scores, linearity, limit of detection, and separation of signal data, show the flexibility and reliability of the Equator HTS, and Chroma-Luc dual reporter technology in any high-throughput situation. 167

TP31<br />

Sophia Liang<br />

Aurora Biomed<br />

Vancouver, Canada<br />

sophia@aurorabiomed.com<br />

Where Laboratory Technologies Emerge and Merge<br />

Co-Author(s)<br />

David Wicks<br />

Joy Goswami<br />

Dong Liang,<br />

Aurora Biomed Inc.<br />

Optimization of A Robotic System For Automation Of Peptide Array Printing<br />

Printing high-density arrays have become an important tool in drug screening, molecular biology, and genetic analysis. Printing multiple<br />

samples onto a solid substrate allows researchers to efficiently screen thousands of conditions in a very small space, thereby saving<br />

time and money. In order to screen for peptides that inhibit bacterial growth, Aurora Biomed Inc. performed array printing onto a solid<br />

substrate using variations of a peptide sequence with its versatile VERSA1000 liquid handling workstation. In order to optimize the<br />

nano-array technology and print volumes, careful standardization procedures were carried out on automation parameters such as pulse<br />

length, pressure, solvent concentration, solvent composition, substrate surface treatment, robotic movement and speed. Results from our<br />

experiments conclusively showed that the optimization was successful in generating high-density arrays for antibodies and peptides at<br />

volumes as low as 15nL on the epoxy coated glass slides. We present here, the optimization procedure of our present printing technology<br />

and explore the applications of the VERSA1000 in array printing.<br />

TP32<br />

David Lorenz<br />

Deerac Fluidics<br />

Dublin 2, Ireland<br />

david.lorenz@deerac.com<br />

Co-Author(s)<br />

Aoife Gallagher, Deerac Fluidics<br />

Brad Larson<br />

Tracy Worzella<br />

Michael Bjerke<br />

Promega Corporation<br />

Eric Matthews, BMG Labtech<br />

High-Throughput Automation of a Dual Reporter Assay in Low-Volume 384 & 1536-<br />

Well Plate Formats Using the Deerac Fluidics’ EquatorTM HTS- Eight Tip Pipetting<br />

System, Promega’s Chroma-Luc Technology, and BMG LABTECH’s PHERAstar<br />

Microplate Reader<br />

The drive towards miniaturization within the pharmaceutical and biotechnology fields has created a need for liquid handling technologies<br />

that accurately deliver low volume reagents to high-density plates. This has also created a need for simple, fully scaleable assays in<br />

low volumes. Here we demonstrate the successful combination of both through the use of the Equator HTS - Eight Tip Pipetting<br />

System, and the dual color Chroma-Luc technology. Cellular lysates, containing the green CBG99luc and red CBRluc genes, followed<br />

by Chroma-Glo reagent, were dispensed in low-volume 384, and 1536-well formats using volumes ranging from 10ul to 500nl.<br />

Luminescence from the two luciferases was then simultaneously measured using the BMG LABTECH PHERAstar plate reader. The<br />

exceptional Z’ Factor scores, linearity, limit of detection, and separation of signal data, show the flexibility and reliability of the Equator<br />

HTS, and Chroma-Luc dual reporter technology in any high-throughput situation.<br />

167

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