LabAutomation 2006 - SLAS

TP29
Hanh Le
PerkinElmer Life and Analytical Sciences
Boston, Massachusetts
hanh.le@perkinelmer.com
LabAutomation2006
Co-Author(s)
Harry Harney
Stephen Hurt
Robert Stanaker
PerkinElmer Life and Analytical Sciences
Rajesh Manchanda
Utilization of the ATPlite 1step Detection System for Homogenous Automated
Cytotoxicity Assays
A number of stategies are currently being implemented in the effort to increase the success rate in identifying new potential therapeutic lead
compounds, and reducing the number of late stage failures in clinical trials. One approach is to perform ADME/Tox profiling at an earlier
stage in the drug discovery process, leading therefore to the need for increased efficiency in carrying out these asssays. ATPlite 1step is
a homogenous, luciferase-based luminescence assay system for the quantitative measurement of proliferation and cytotoxicity in cultured
mammalian cells. The assay is based on the measurement of ATP as a marker for cell viability, and is both highly sensitive and easy-to-use.
The single addition format for the assay makes it particulary suitable for automation. We have used the ATPlite 1step system to perform
automated cytoxicity assays on the Cellular Workstation, an integrated walk-way system for cellular assays. The components of the
workstation as configured for this assay include an Evolution P3 for reagent dispensing, EnVision microplate reader,CataLyst Express
robotic arm, Cytomat microplate incubator and POLARATM scheduling sorftware. Data will be presented demonstrating the assay protocol
and the automation methodology.
TP30
Anthony Lemmo
Entevis Inc
Sudbury, Massachusetts
tlemmo@entevis.com
Automated Dispensing of Solid Powders and Viscous Reagents: Enabling Solutions for
Material Discovery, Development and Optimization
Two major challenges in the automation of aspects of new materials development are the dispensing of solid powders and dispensing
high viscosity reagents. We have developed automated, benchtop systems to address both of these key challenges. The solid dispensing
system is capable of dispensing powders with bulk densities ranging from 0.03 to 3 in the mass range from 100 micrograms to hundreds
of milligrams with %CV’s of 15% or better. The viscous fluid dispensing system can handle reagents with viscosities that range from 0.01
to 900 cp and cover the volume range from 1 microliter to hundreds of milliliters with %CV’s typically < 2%. These systems, either used
as stand-alone solutions, or in a combined workstation, have utility in the pharmaceutical development process for the automation of
aqueous solubility, salt selection, polymorph screening and animal dosing experiments. They can also have a major impact in compound
management operation, where the majority of samples are either solid powders or “difficult” liquids (e.g. oils, waxes, etc.).
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