LabAutomation 2006 - SLAS
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LabAutomation 2006 - SLAS

LabAutomation 2006 - SLAS LabAutomation 2006 - SLAS

slas.org
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TP25 Joseph Kofman Pfizer San Diego, California joseph.kofman@pfizer.com LabAutomation2006 Co-Author Todd Baumgartner Embracing the Unattainable: Creating an Integrated Electronic Environment for Analytical Laboratories Even a superficial review of working practices within analytical laboratories across the pharmaceutical industry reveals key areas within the workflow that have unmet needs with respect to integrated informatics systems. Despite tremendous efforts to move towards a “paperless” environment, current systems within analytical laboratories typically provide autonomous support in relation to other analytical processes. Knowledge is successfully captured and accumulated, but not shared effectively. The development and implementation of a complete “chain of custody” solution for laboratory data and information is needed to fully meet the compliance requirements of 21 CFR Part 11, provide extended productivity improvements, and reduce the support and maintenance costs of multiple redundant systems. There has been work in integrating different solutions (e.g. CDS and LIMS); however the strategy for a complete system has not been defined. A critical aspect of the Analytical Laboratory Integrated Solution (ALIS) is that each of the systems is linked through touch points (common interfaces), and all laboratory data is acquired and stored electronically by specialty applications, but is accessible for data mining through all associated applications. A prototype of ALIS was built based on a strategic process map and focused on integration and information exchange between independent applications. The integrated solution was designed so that information flow within the analytical laboratory was improved or at least maintained. The benefits gained from this integrated system are far greater than if the components were implemented independently. TP26 Saikalyan Kotha Flow Sciences Leland, North Carolina skotha@flowsciences.com Co-Author(s) Ray Ryan Douglas B. Walters, KCP Inc. Ventilated Robotic Enclosures for Product and Personal Protection in High-Throughput Laboratories Today’s potent material handling laboratories have changed significantly. Synthesis-based R&D has moved into the new millennium and been supplemented with processes using sophisticated computer and high throughput robotic technology. The laboratory use of novel compounds of unknown potency is rapidly expanding, and requires flexible task-specific containment solutions to minimize environmental impact, protect operators, and optimize process efficiency. While many laboratory operations can only be safely performed in large traditional chemical hoods, or biological safety cabinets limitations often arise because containment is not always effective, the hood design is not task-specific and is not designed for high through put equipment, relocation is difficult, and purchase, installation and operation is expensive. Hence, in many cases unique process-specific containment solutions must be developed to provide safe, adaptable and energy efficient enclosures in the rapidly changing laboratory environment. This presentation describes several custom designed vented enclosures developed for pharmaceutical and medical research. This project encompassed three distinct phases critical to the project’s successful completion. The definition of specific robotic equipment such as, liquid handling, incubators, and powder handling equipment. Optimizing designs with computational fluid dynamics (CFD) to maximize containment and energy efficiency, and to resolve ergonomic issues and provide easy operator access to the enclosed equipment. Commissioning of enclosures with on-site testing to ensure effective containment. This project emphasizes the importance of task-specific containment solutions for use with high-through put and robotic equipment 164

TP27 Steve Lappin Amgen Thousand Oaks, California slappin@amgen.com Where Laboratory Technologies Emerge and Merge Co-Author Alex Mladenovic, Amgen Inc. A Simple and Compact Liquid Handler/Centrifuge Integration Many biological assays and sample preparation protocols require separation of solids from liquid for processing, often accomplished by centrifugation. As centrifuge integrations into automated platforms are typically large and complex and require another component to shuttle plates, they are often not appropriate for smaller labs or lower throughput applications and can be prohibitively expensive. We have developed and implemented compact platform consisting of an integrated Velocity11 Vspin centrifuge (and Access unit) with a Beckman Biomek NX that requires no additional components to load the centrifuge. We have written a rudimentary driver to control the centrifuge directly from the Biomek software and is applicable to all Biomek software versions. The entire device measures only 0.9M x 1.3M and is appropriate for many applications that require washes and spins, such as flow cytometry sample preparation, blood preparations and antibody labeling experiments. TP28 Brad Larson Promega Corporation Madison, Wisconsin brad.larson@promega.com Co-Author(s) Tracy Worzella Promega Corporation Siegfried Sasshofer Tecan Aoife Gallagher Deerac Fluidics Automated Multiplexed Cell-Based Assays for High-Throughput Drug Discovery Today’s high-throughput screening facilities face increasing demands to generate more information from existing compound libraries. An appealing solution is to perform multiplexed assays during the same cell-based screen. A multiplexed-assay approach allows for the evaluation of multiple parameters from the same sample source. An overall decrease in screening costs and variability are also realized when different assays can be used on the same plate of cells. Here we provide proof-of-principle data by combining Promega’s cell-based screening assays in a multiplexed format with a variety of high-throughput instrumentation. Live cell reporter, cell viability, and caspase – 3/7 activity assays were combined in 96, 384, and 1536-well formats. A unique combination and detection of both luminescent and fluorescent chemistries within the same well is also demonstrated. The single-reagent additions to each well, extended signal half-lives and sensitivity of each of these compatible chemistry combinations make them ideal for high-throughput liquid handling and detection. 165

TP27<br />

Steve Lappin<br />

Amgen<br />

Thousand Oaks, California<br />

slappin@amgen.com<br />

Where Laboratory Technologies Emerge and Merge<br />

Co-Author<br />

Alex Mladenovic, Amgen Inc.<br />

A Simple and Compact Liquid Handler/Centrifuge Integration<br />

Many biological assays and sample preparation protocols require separation of solids from liquid for processing, often accomplished by<br />

centrifugation. As centrifuge integrations into automated platforms are typically large and complex and require another component to<br />

shuttle plates, they are often not appropriate for smaller labs or lower throughput applications and can be prohibitively expensive. We have<br />

developed and implemented compact platform consisting of an integrated Velocity11 Vspin centrifuge (and Access unit) with a Beckman<br />

Biomek NX that requires no additional components to load the centrifuge. We have written a rudimentary driver to control the centrifuge<br />

directly from the Biomek software and is applicable to all Biomek software versions. The entire device measures only 0.9M x 1.3M and<br />

is appropriate for many applications that require washes and spins, such as flow cytometry sample preparation, blood preparations and<br />

antibody labeling experiments.<br />

TP28<br />

Brad Larson<br />

Promega Corporation<br />

Madison, Wisconsin<br />

brad.larson@promega.com<br />

Co-Author(s)<br />

Tracy Worzella<br />

Promega Corporation<br />

Siegfried Sasshofer<br />

Tecan<br />

Aoife Gallagher<br />

Deerac Fluidics<br />

Automated Multiplexed Cell-Based Assays for High-Throughput Drug Discovery<br />

Today’s high-throughput screening facilities face increasing demands to generate more information from existing compound libraries.<br />

An appealing solution is to perform multiplexed assays during the same cell-based screen. A multiplexed-assay approach allows for the<br />

evaluation of multiple parameters from the same sample source. An overall decrease in screening costs and variability are also realized<br />

when different assays can be used on the same plate of cells. Here we provide proof-of-principle data by combining Promega’s cell-based<br />

screening assays in a multiplexed format with a variety of high-throughput instrumentation. Live cell reporter, cell viability, and caspase<br />

– 3/7 activity assays were combined in 96, 384, and 1536-well formats. A unique combination and detection of both luminescent and<br />

fluorescent chemistries within the same well is also demonstrated. The single-reagent additions to each well, extended signal half-lives and<br />

sensitivity of each of these compatible chemistry combinations make them ideal for high-throughput liquid handling and detection.<br />

165

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