LabAutomation 2006 - SLAS

TP23
Tanya R. Knaide
ARTEL
Westbrook, Maine
tknaide@artel-usa.com
Where Laboratory Technologies Emerge and Merge
Co-Author(s)
John Thomas Bradshaw
Benjamin W. Spaulding
Alex Rogers
Ceara McNally
Rapid Volume Verification in High-Density Microtiter Plates Using Dual-Dye Photometry
With a significant movement toward miniaturization in liquid volume dispensing for high-density microtiter plate arrays, the need for a
fast, accurate method for volume verification is imperative. The increase in high-throughput screening for microtiter plate assays is solely
based on the increased use of automated liquid handling (ALH) systems. Because of this increase, there is a tremendous need for volume
verification methodology which does not require highly-trained technicians or significantly slow screening processes. Presently, there are
very few methodologies that can quickly and accurately interrogate dispensed volume in individual wells, within a high-density microtiter
plate. Validation of sub-microliter liquid volumes in 384-well plates is an extremely challenging task with dramatically increasing regulatory
importance. Current volume verification methods are subject to external influences such as evaporation, static and incomplete mixing. The
ALH systems employed require consistently optimum performance in order to prevent instrument down-time and loss of productivity.
The dual-dye photometric technology employed by Artel’s Multichannel Verification System (MVSTM) overcomes challenges associated
with determining the dispensed volume within each well of a 384-well microtiter plate. MVS extends it’s currently accepted volume
measurement approach for 96-well plates to 384-well plates and volumes as low as 30 nanoliters. The measurements collected by the
MVS in less than 15 minutes provide both precision and accuracy values per individual channel. The system capabilities enable easy
optimization of small volume dispense protocols to minimize valuable sample waste. This presentation will discuss the system technology,
validation of the method utilized and ALH protocol optimization using the MVS.
TP24
Steffen Koehler
EVOTEC AG
Hamburg, Germany
steffen.koehler@evotecoai.com
Compound Management at Evotec AG – be Flexible
Compound logistics centers of biotech and pharmaceutical companies serving HTS facilities face demands for increasing throughput and
flexibility in terms of plate types, plate layouts and types of HTS systems. Specially for a contract screening service, it is also particularly
important to ensure the secure separation of customer libraries and to deal with all the different customer needs for e.g. plate types, plate
layouts, amount of compound, and data formats.
How does Evotec AG solve the challenge? Which software and hardware is involved? Which screening systems are installed and how is
everything linked together? What happens from the reception of customer compound libraries until the screening data are transferred to
our customers?
Evotec AG shows a solution for a fast, flexible, highly reliable, auditable and safe compound management to meet the demands for the
next years.
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