LabAutomation 2006 - SLAS
LabAutomation 2006 - SLAS
LabAutomation 2006 - SLAS
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TP17<br />
Michael Gary Jackson<br />
Beckman-Coulter<br />
Fullerton, California<br />
mgjackson@beckman.com<br />
<strong>LabAutomation</strong><strong>2006</strong><br />
Co-Author(s):<br />
Matthew Cu<br />
Sunghae Joo<br />
Han-Chang Chi<br />
Keith Roby<br />
Scott Boyer,<br />
Beckman-Coulter<br />
Automation of Gene Expression on Beckman Coulter’s Biomek ® 3000 Laboratory<br />
Automation Workstation<br />
Manual preparation of materials through the complex gene expression chemistry process can introduce variation in samples and potentially<br />
distort data output. Even simplified processes with higher numbers of samples would also be likely to suffer from such inaccuracies.<br />
Automated processing of samples provides consistency in results for the steps from RNA preparation and cDNA setup through PCR1<br />
setup. Consistency in sample preparation is essential to be able to monitor the variations in cellular signals characteristic of biological<br />
systems. Automation is based on the Biomek 3000 and requires no changes in the deck configuration through the sample preparation<br />
process. We evaluated the sample preparation of total RNA from treated and untreated cell cultures for both human and rat cell lines.<br />
The samples were then processed through automation methods that are part of the GenomeLab GeXP application. Both singlet and<br />
multiplex PCR primer sets were used for comparison of transcript levels. We also included intra-plate samples to show consistency and<br />
reproducibility of the process in comparison to the manual process.<br />
TP18<br />
Joby Jenkins<br />
TTP LabTech<br />
Royston, Hertfordshire,<br />
United Kingdom<br />
joby.jenkins@ttplabtech.com<br />
Co-Author(s)<br />
Rob Lewis<br />
Tristan Cope<br />
Wayne Bowen<br />
TTP LabTech<br />
Automated Nanolitre Hit-Picking Using A mosquito ® X1 Low Volume Pipettor<br />
A prerequisite for efficient primary screening is the automated selection of “hits” for confirmation and secondary profiling. Solutions exist for<br />
low density microplates, however, the 1,536 well format presents significant challenges for many liquid handling systems. The mosquito ®<br />
X1 offers precision sampling of any individual well in 48-, 96-, 384- or 1,536-well plates. This enables researchers to quickly select small<br />
volumes of “hits” from primary screening plates and transfer them directly to the next screening stage without further dilution. mosquito ®<br />
X1’s disposable pipette tips guarantee zero cross-contamination. The system’s unique positive displacement pipetting technology allows<br />
it to pipette accurately and reproducibly throughout the 25nl-1.2ìl range, producing CVs of
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