LabAutomation 2006 - SLAS
LabAutomation 2006 - SLAS
LabAutomation 2006 - SLAS
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TP13<br />
Ulrike Honisch<br />
Greiner Bio-One<br />
Frickenhausen, Germany<br />
Ulrike.Honisch@gbo.com<br />
<strong>LabAutomation</strong><strong>2006</strong><br />
Co-Author(s)<br />
Rainer Heller, Greiner Bio-One GmbH<br />
Dagmar Weber, Evotec Technologies GmbH<br />
Inka Pfitzner, Biotesys GmbH<br />
Stefan Marose, Evotec Technologies GmbH<br />
High Content Analysis of Biochemical and Cellular Assays in SensoPlate<br />
Plus Glass Bottom Microplates<br />
Integrated and automated workstations do set new standards for ease of use and sophisticated fluorescence techniques. For various<br />
applications in drug discovery including confocal single molecule detection, high performance microplates are mandatory, when looking<br />
for superior data quality. To meet this need Greiner Bio-One designed in cooperation with evotec technologies new microplates for High<br />
Content Screening applications. The CellCarrier microplates are tissue culture treated polystyrene film bottom microplates adapted to<br />
the needs of cellular assays. The SensoPlate Plus microplates are novel glass bottom microplates especially recommended for use in<br />
fluorescence correlation spectroscopy (FCS) and related confocal methods (FCS+plus). The optimized microplate geometry of the Cell<br />
Carrier and the SensoPlate Plus permits complete utilization of all wells even for measurements with immersion objectives. We tested<br />
the SensoPlate Plus microplates in different biochemical and cellular assays. Due to the high optical quality of the glass bottom and the<br />
minimal bending of less than 100 µm SensoPlate Plus shows homogenous readouts with CV’s below 2.5, high count rates and high Z’<br />
values. Therefore SensoPlate Plus is particularly suitable for biochemical and non-adherent cell assays. Cell growth of adherent cells on<br />
glass bottom microplates is more difficult to assess. The performance of SensoPlatePlus, different coatings and polystyrene microplates<br />
in cell based assays will be discussed.<br />
TP14<br />
Peter F. Huefner<br />
Merck & Co., Inc.<br />
Rahway, New Jersey<br />
peter_huefner@merck.com<br />
Co-Author(s)<br />
J. Chris McWilliams<br />
Chaoxian Cai<br />
High Throughput Screening and Optimization of Catalytic Reactions in the Catalysis<br />
Lab at Merck<br />
This poster will present the technology being employed at Merck, using specific examples wherein automation accelerated discovery and<br />
process development. Particular emphasis will be placed on examples in asymmetric hydrogenations<br />
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