LabAutomation 2006 - SLAS

TP09
Aoife Gallagher
Deerac Fluidics
Dublin 2, Ireland
aoife@deerac.com
LabAutomation2006
Co-Author(s)
Joe Graham
Sheila Fisher
Tracey Honan
Susan Faro
Broad Institute
Miniaturization of Liquid Handling Procedures in High Throughput Sequencing at the
Broad Institute.
Deerac Fluidics Equator products are designed to fit a wide range of applications requiring low volume liquid handling. The products
have recently been installed as part of the high throughput sequencing production process at the Broad Institute in Boston, MA, USA
(formerly the Whitehead Center for Genome Research). The Genome Sequencing and Analysis program at the Broad Institute places
emphasis on the development of scalable methods employing automated laboratory procedures and informatics systems. The current rate
of sequencing corresponds to over 40 million lanes per year, deployed in sequencing the human, mouse, and other genomes. This poster
will describe the current process employed by Broad Institute, highlighting where the Equator has played a vital role in performance
enhancement and cost reduction, enabling the Broad Institute to retain its position as the world’s leading genome research institute.
TP10
Huidong Gu
Bristol-Myers Squibb
Princeton, New Jersey
huidong.gu@bms.com
Co-Author(s)
Steve Unger
Yuzhong Deng
Bristol-Myers Squibb
Fully Automated Sample Preparation by Using Tecan for LC/MS/MS Assay
In an accelerated drug development process, large numbers of samples from the clinical studies need to be quantified for their concentrations
in a variety of biological fluids using validated bioanalytical assays. To enhance the throughput, a Tecan RSP liquid handling system is used
for automated sample preparation in our lab as it provides a friendly user interface for Tecan programming. However, analytical assays
change on daily basis. It is time consuming and error prone for users to write or modify Tecan programs every day. EZTecan is a program
designed to help users overcome this problem.
In the drug development process, Watson LIMS was used to support DMPK/Bioanalytical studies. EZTecan can directly convert the
information list in Watson to a Tecan program for sample preparation, including sample transfer, dilution, SPE (or PP), and reconstitution. A
custom designed worktable layout was applied to the Tecan system to save the liquid handler trips for sample transfer and extraction.
Several bioanalytical LC/MS/MS assays were validated by using the EZTcan program to control the Tecan liquid handler system. This
approach demonstrates a fast, accurate and reliable procedure for sample preparation. It takes about 90 min to prepare two full 96-well
plates from sample transfer to reconstitution. Analytical results shown good inter and intra-assay accuracy and precision which met the
GLP acceptance criteria. EZTecan is very easy to learn and use and bioanalysts can use Tecan without writing a single line of Tecan
program for various assays on daily base.
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