LabAutomation 2006 - SLAS
LabAutomation 2006 - SLAS
LabAutomation 2006 - SLAS
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MP95<br />
Christine Brideau<br />
Merck Frosst Centre for Therapeutic Research<br />
Kirkland, Quebec, Canada<br />
christine_brideau@merck.com<br />
<strong>LabAutomation</strong><strong>2006</strong><br />
Co-Author(s)<br />
Sébastien Guiral<br />
Frédéric Massé<br />
Merck Frosst Centre for Therapeutic Research<br />
Jeffrey Karg<br />
Doug Kroncke<br />
nAscent Biosciences Inc.<br />
Nanoliter Dispensing of Compounds into Assay Plates Using Disposable PocketTipsTM<br />
Successful assay miniaturization for screening requires low volume transfer of test compounds dissolved in DMSO. Due to the low DMSO<br />
tolerance of most assays, many laboratories are required to make intermediate solutions in aqueous buffer. This may result in compound<br />
precipitation prior to compound dispensing into the assay plate. To circumvent this problem, we evaluated 100nL compound transfer using<br />
the Biomek FX 96 and 384-head compatible PocketTips. Reproducibility, precision and accuracy will be presented using fluorescent<br />
dyes and a panel of test compounds. Results from 2 enzymatic assays will be shown and the issue of ‘sticky’ hydrophobic compounds will<br />
be discussed.<br />
MP96<br />
Marc Pfeifer<br />
Roche Molecular Systems, Inc.<br />
Pleasanton, California<br />
marc.pfeifer@roche.com<br />
Co-Author(s)<br />
Josh Weinberger<br />
Dan Bristol<br />
Mike Takeuchi<br />
Paulette Thomas<br />
Imre Trefil<br />
Jon Nunes<br />
The Cobas s 201 System: Modular Automation for NAT Blood Screening of HCV, HBV,<br />
HIV, and West Nile Virus<br />
The Roche cobas s 201 System running the cobas TaqScreen Multiplex (MPX) Test and the cobas TaqScreen West Nile Virus (WNV) Test<br />
is an automated NAT system for screening donated blood. The system is capable of handling pooled plasma specimen testing, as well<br />
as single unit resolution testing. Utilizing real-time PCR technology for nucleic acid amplification and detection can effectively help identify<br />
donations in the pre-seroconversion “window period”. The basic configuration of the modular cobas s 201 consists of a Hamilton Microlab<br />
Star instrument for pool pipetting, a COBAS AmpliPrep instrument for automated sample preparation, and a COBAS TaqMan instrument<br />
for PCR amplification and detection. A central host interface computer system running Pooling and Data Management (PDM) software<br />
is used for automated results compilation and reporting. Pooling, data management, and PCR result handling are integrated. The entire<br />
testing process for a donor is tracked in the database. Non-reactive test results are transferred to the associated donors automatically for<br />
reporting. Results requiring resolution, i.e. invalid results or valid Reactive results of a pool, automatically trigger a new pooling action to be<br />
performed by an operator. Final results are exported to a host LIS system. The full lifecycle of a donor from initial pool until final PCR result<br />
is tracked.<br />
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