LabAutomation 2006 - SLAS

MP93
Charles Ufomadu
California State University, Los Angeles
Culver, California
cufomadu@yahoo.com
Where Laboratory Technologies Emerge and Merge
Co-Author
Frank A Gomez
Low Pressure Microfluidic-Capillary Electrophoresis (CE) Separations
The past 10 years have witnessed tremendous advances in the design and use of microelectromechanical systems (MEMS). Applications
for microfluidic devices (MDs) have proliferated at a speed reminiscent of the explosive use of microelectronics after the integrated circuit
was invented. One area that microfluidic systems have been used successfully is in capillary electrophoresis (CE) and specifically in
chemical separations. Unlike HPLC techniques, CE utilizes differences in charge-to-mass ratios to afford separation of chemical species.
CE and microfluidic systems are congruent in terms of volume requirements and the array of detection modes that can be utilized in a
given application. The development of a high-throughput technique that couples both CE and microfluidics would be of great interest to
scientists eager to exploit the power of both techniques. Herein, we report the use of MDs constructed by multilayer soft lithography (MSL)
and poly(dimethylsiloxane) (PDMS) in the separation of small biological materials. By automating the sample introduction method whereby
pressure and voltage are coupled to each other, separation of chemical species is expedited as compared to traditional commercial
instruments.
MP94
Maria-Dawn Lilly
BD Biosciences
Bedford, Massachusetts
maria-dawn_lilly@bd.com
Co-Author(s)
Michael Shanler
Michael Ardiff
Tim Ciolkosz
Maurice Kashdan
Christine Aubin
Joseph Goodwin
BD Biosciences
A Fully Automated Workstation for Testing Flow-Based Kinetic Solubility of Compounds
An automated flow-based solubility workstation has been created by integrating the BD Gentest Solubility Scanner to a TECAN Freedom
EVO ® 100 fluid handling workstation. This workstation enables a “walk-away” solution for unattended operation. A robot method is outlined,
which includes the following: creating compound plates from vials; serially diluting more than 10 concentration wells; bar code scanning
to track compound data; transferring plates between the scanner and liquhandler; and running a method on the scanner. The workstation
deck layout is outlined, and a method for optimized liquid handling and analysis is described. Additionally, data from 22 compounds are
compared using manual versus automated sample preparation methods. High sensitivity for accurate readings of low solubility compounds
is shown, as well as data on compound solubility