LabAutomation 2006 - SLAS

MP81
Steven Wilson
Joint Genome Institute
Walnut Creek, California
sewilson@lbl.gov
Where Laboratory Technologies Emerge and Merge
Co-Author(s)
Paul Richardson
Feng Chen
Jamie Jett
Nancy Hammon
Duane Kubischta
Diana Lawrence
Implementing the Agencourt SprintPrep384 Protocol at JGI
SprintPrep DNA isolation is a process that allows large fragments of DNA and vectors to be isolated from the host E. Coli cell. Agencourt
has developed SprintPrep reagents and semi-automated methods for performing the necessary protocol. Last year, JGI implemented a 96
well SprintPrep method. This year, JGI has made the 384 SprintPrep method virtually user-independent.
Moving from the 96 well fosmid isolation method to the 384 well format has led to cost savings due to reagent reductions and a doubling in
sequencing throughput. The increase in throughput will lead to an increase in sequencing depth and data confidence.
This work was performed under the auspices of the US Department of Energy’s Office of Science, Biological and Environmental Research
Program and the by the University of California, Lawrence Livermore National Laboratory under Contract No. W-7405-Eng-48, Lawrence
Berkeley National Laboratory under contract No. DE-AC02-05CH11231 and Los Alamos National Laboratory under contract No. W-7405-
ENG-36.
MP82
Lester Wong
Alberta Agriculture
Edmonton, Alberta, Canada
lester.wong@gov.ab.ca
Co-Author(s)
John Wu
Evelyn Bowlby
Alberta Agriculture
An Automated High-Throughput Screening Enzyme Linked Immunosorbent Assay for
Johne’s Disease Antibodies in Bovine Serum
One manual Mycobacterium paratuberculosis enzyme linked immunosorbent assay (ELISA) was fully automated using an Automation
Workstation System designed based on a modular approach. This high throughput screening configuration consisted of a Beckman
Coulter hybrid Biomek ® FX liquid handling system integrated with a Molecular Devices Spectramax ® microplate reader, a Biotek ® ELx405
microplate washer and a Kendro Cytomat ® 6000 hotel. The integrated system function was programmed with an IBM computer using the
SAMI ® software. This setup was housed in a Class 2 Biosafety Cabinet. The two assays were validated according to the requirements of
the ISO/IEC 17025 standard. Starting in 2001, the test scope has received ISO accreditation from Standard Council of Canada. We have
been approved by the United States Department of Agriculture to perform Johne’s disease serology using the ELISA since 1998. The
productivity of full automation of this test has been discussed. The performance characteristics, such as the kappa quotient, identity score,
test sensitivity and specificity, positive and negative predictive values of 2 commercial manual tests (IDEXX and BIOCOR) were evaluated.
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