LabAutomation 2006 - SLAS
LabAutomation 2006 - SLAS
LabAutomation 2006 - SLAS
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MP75<br />
Lois Tack<br />
PerkinElmer Life & Analytical Sciences<br />
Downers Grove, Illinois<br />
lois.tack@perkinelmer.com<br />
<strong>LabAutomation</strong><strong>2006</strong><br />
Co-Author(s)<br />
Peng Li<br />
Karen Gecic<br />
PerkinElmer Life and Analytical Sciences<br />
An Automated DNA Purification and Quantification Solution Using the JANUS<br />
Automated Workstation With Integrated Victor3 Plate Reader<br />
An integrated automated nucleic acid purification and analysis robotic workstation will increase sample throughput and turnaround time<br />
while decreasing user error and the variability often associated with manual processing. The JANUS Automated Workstation platform from<br />
PerkinElmer Life and Analytical Sciences is designed to be a complete application solution for a wide variety of nucleic acid extraction and<br />
purification protocols. These include both vacuum filtration/solid phase extraction and magnetic bead separation chemistries.<br />
We describe here the fully automated rapid purification and quantification of genomic DNA from cell samples using the Promega Wizard®<br />
SV 96 Genomic DNA Purification System and PicoGreen® dsDNA Quantitation Reagent. The JANUS workstation consisted of a Two Arm<br />
Integrator Platform (8-tip Varispan, Gripper), Vacuum Filtration and Shaker Options. The application–specific software template utilizes<br />
the Promega kit reagents and filter plates to prepare high quality genomic DNA followed by automated fluorescent quantitation and<br />
normalization using the multi-mode Victor3 Plate Reader with the Workout Data Analysis Software Option.<br />
MP76<br />
Eric Tan<br />
Keck Graduate Institute<br />
Claremont, California<br />
etan@kgi.edu<br />
Co-Author(s)<br />
Ekaterina Kniazeva<br />
Jennifer Wong<br />
Krisanu Bandyopadhyay<br />
Angelika Niemz<br />
New Methods for Rapid Isothermal Amplification and Detection of Short DNA Sequences<br />
We have coupled a novel isothermal amplification method for short oligonucleotides (EXPAR) with detection involving DNA-functionalized<br />
gold nanospheres in solution or on surfaces. In these assays, a trigger oligonucleotide is exponentially amplified and converted to a reporter<br />
oligonucleotide capable of aggregating DNA-functionalized gold nanospheres in solution or immobilizing them on probe functionalized<br />
surfaces. The solution based assay relies on simple visual detection mitigated by the color change from red to dark purple or blue<br />
upon DNA nanosphere aggregation, which is detectable through spotting of the solution onto a suitable substrate. The overall assay is<br />
sequence-specific, and permits detection of 100 fM trigger in less than 10 minutes with minimal requirement for instrumentation. We have<br />
developed a multiplexed version of the reaction suitable for surface-based assays in a low-density microarray format using either optical or<br />
electronic means.<br />
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