LabAutomation 2006 - SLAS

MP73
Michael Stangegaard
Technical University of Denmark
Kongens Lyngby, Denmark
mst@mic.dtu.dk
Where Laboratory Technologies Emerge and Merge
Co-Author(s)
Sarunas Petronis
Chalmers Tekniska Högskola
Claus B. V. Christensen
Coloplast Denmark
Martin Dufva
Technical University of Denmark
A Biocompatible Micro Cell Culture Chamber (µCCC) for Culturing and Online
Monitoring of Mammalian Cells.
Cell culturing in a micro cell culture chamber enables the online monitoring of cellular events and morphological changes in real-time
during cell proliferation. Exchanging the culture surface of the reference cell culture flask with a different surface can pose biocompatibility
problems resulting in altered gene expression profiles, growth kinetics or apoptosis. Crucial to biological investigations using micro
reactors or different surfaces rather than the standard cell culture flask is that the cells experience a similar environment and hence provide
corresponding biological reaction parameters. We have demonstrated that cell culturing on PMMA does not change the gene expression
profile of HeLa cells using full transcriptome 60bp oligo DNA microarray. We then realized a micro cell culture chamber (µCCC) in PMMA
by laser ablation and thermal bonding relying on continuous perfusion of media, and on chip thermal heat regulation enabling culturing of
mammalian cells directly on a microscope stage. Culturing for at least two weeks with real-time monitoring is demonstrated. Comparing
the gene expression profile of cells cultured in the µCCC with reference cells cultured in a conventional culture flask suggested that the
µCCC provides a culture environment indistinguishable from the cell culture flask.
MP74
Joni Stevens
Gilson, Inc.
Middleton, Wisconsin
jstevens@gilson.com
Co-Author(s)
Greg Robinson
Alan Hamstra
A Novel Approach for the Isolation and Concentration of Drugs in Biological Fluids via
On-Line Dialysis and Enrichment
Sample preparation is a necessary for the analysis of drugs/compounds in biological fluids. Many techniques are available from protein
precipitation through solid phase extraction, however all sample preparation techniques have limitations, from the simplest, drying down
the eluent to the more complex, method development for solid phase extraction. On-line dialysis and trace enrichment prior to analysis
offers an approach to sample preparation that eliminates many of these issues. The automated system introduces a biological sample
to a dialysis membrane and through positive fluidics separates the drugs/compounds from the matrix and concentrates it onto a trace
enrichment cartridge prior to analysis via HPLC. The system operates parallel to the chromatography system; therefore no time is lost to
sample preparation. Data will be presented that verify the usefulness of this sample preparation technique for the analysis of drugs from
biological fluids
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