LabAutomation 2006 - SLAS

MP63
Qiaosheng Pu
Virginia Commonwealth Univeristy
Richmond, Virginia
qpu@vcu.edu
LabAutomation2006
Co-Author(s)
Bowlin Thompson
Julio C Alvarez
Photochemical Modification of Cyclic Olefin Copolymer Microfluidic Chips for
Biomolecule Microarrays and Surface Property Patterning
Here we report the surface functionalization of plastic microfluidic channels to control microchip surface properties and to prepare
biomolecule arrays. The modification is attained by a photoinduced grafting reaction in which acryl monomers along with a photoinitiator
react on the exposed surface to produce a polymer film anchored to the plastic surface. Cyclic Olefin Copolymer (COC) was selected
among other commodity thermoplastics because of its higher UV transparency which allows on-chip modification of the surface in
selected locations of the microchip. Using this method it is possible to graft thin polymer films containing amine and carboxylate groups
that can be further derivatized to incorporate the streptavidine-biotin chemistry. In this way many commercially available bioconjugates
can be immobilized in selected areas of the chip. The immobilized reagents will act as capture probes in chemical affinity analysis (DNA
hybridization, immunoassays, enzyme reactions, etc.), or merely as ways of imparting specific charge to the surface for controlling flow.
The polymer films were characterized in open surfaces using fluorescence, profilometry, electrokinetic methods and infrared spectroscopy.
MP64
Charles Reichel
EDC Biosystems
San Jose , California
creichel@edcbiosystems.com
Co-Author
Michael Forbush
Effect of Focal Distance on Drop Volume in Acoustic Drop Ejection
Acoustic drop ejection is the process of producing drops by focusing a beam of acoustic energy at the surface of a liquid and allowing the
pressure wave to force the fluid out of the container. In this process, the shape of the focused beam at the surface, the energy used, and the
length of time the energy is applied will determine the volume of liquid ejected. Since the shape of the focused beam at the surface changes
with focus, it is important to understand the dependence of focus on drop volume dispensed. In this study the sensitivity of focus on drop
volume is explored. In addition, the sensitivity of the pressure pattern on the surface with respect to focal distance is also important to the
natural distribution of drop volume ejected. In this study, the distribution of drop volume ejected with respect to focal distance is also studied.
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