LabAutomation 2006 - SLAS
LabAutomation 2006 - SLAS
LabAutomation 2006 - SLAS
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MP59<br />
Irena Nikcevic<br />
University of Cincinnati<br />
Cincinnati, Ohio<br />
nikcevi@email.uc.edu<br />
<strong>LabAutomation</strong><strong>2006</strong><br />
Co-Author(s)<br />
Se Hwan Lee<br />
Aigars Piruska<br />
Chong H. Ahn<br />
Patrick A. Limbach<br />
William R. Heineman<br />
Carl J. Seliskar<br />
University of Cincinnati<br />
Characterization of Cyclic Olefin Copolymer (COC) and Poly(methylmethacrylate)<br />
(PMMA) Microchips for Capillary Electrophoresis<br />
A high-throughput plastic microchip analytical technology based on capillary electrophoresis (CE) for rapid analysis and manipulation<br />
of biological samples and small molecule therapeutics important for medical and pharmaceutical research is being developed. Using<br />
plastics for fabrication material reduces the cost and eventually will lead to high-speed, mass production of disposable chips. Compared<br />
to glass (the standard material) plastic materials have different properties and thus it is important to characterize their properties before<br />
real biological assays can be performed. When designing a chip in a plastic substrate several issues must be addressed, including the<br />
materials and their properties, the scale of the system, methods of fabrication, detection scheme, and most importantly, stability and<br />
evaluation of analytical performance. The performance characteristics of single lane plastic CE separations were evaluated using mixtures<br />
of the dyes fluorescein and fluorescein isothiocyanate as model compounds. Fabrication properties, quality of fabricated chip, separation<br />
reproducibility, determination of electroosmotic flow (EOF) and electrophoretic mobility of hot embossed poly(methylmethacrylate) (PMMA),<br />
injection molded PMMA and cyclic olefin copolymer (COC) were compared with results obtained for glass chips.<br />
MP60<br />
Aigars Piruska<br />
University of Cincinnati<br />
Cincinnati, Ohio<br />
piruska@email.uc.edu<br />
Co-Author(s)<br />
Irena Nikcevic<br />
Patrick A. Limbach<br />
William R. Heineman<br />
Carl J. Seliskar<br />
University of Cincinnati<br />
Optical Detection System for Multi-Lane Plastic Microchips<br />
High throughput analysis of disease targets and candidate pharmaceuticals are critical for new pharmaceutical discovery and to reveal<br />
insight into disease development. Our research group is developing methodology for high speed assays. Analysis is based on microchip<br />
capillary electrophoresis performed on multi lane disposable plastic chips. A method for simultaneous optical detection of all multi lane<br />
channels is demonstrated. All separation channels on the multi lane microchip are simultaneously excited by a linearly expanded laser<br />
beam and fluorescence from the analyte is detected by a CCD camera. The detailed characterization of the developed detection scheme<br />
was performed. Uniform and efficient excitation over fairly large distances (few millimeters) was achieved by using a Powel lens for laser<br />
beam expansion. The signal from adjacent channels did not show significant crosstalk. The separation performance of a model system is<br />
compared for a standard single lane and a multi lane detection system.<br />
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