LabAutomation 2006 - SLAS

MP55
Philip Manning
Procter & Gamble Pharmaceuticals
Norwich, New York
manning.pj@pg.com
LabAutomation2006
Electronic Data Entry From Microsoft Excel Into an Oracle Based LIMS
In a typical scientific laboratory, thousands of result records are generated each day from various instruments and robots. Raw data
collected are rarely useful in their original form and calculations must be performed with them to get meaningful results. Often only these
calculated results are stored in a Laboratory Information Management System (LIMS). The method to import this data into a LIMS is usually
a time consuming, error prone, manual method that is not a good utilization of the Analysts time or expertise. This paper describes an
electronic data transfer method specifically from Microsoft Excel to an Oracle based LIMS. However, the source of the data would not have
to be Excel - it could be a chromatography application, word processor or any other Windows based application. Benefits of electronic
data entry include: reduced data entry time, increased accuracy, better utilization of resources and increased employee job satisfaction by
eliminating tedious, non-challenging tasks.
MP56
Verónica Martins
Centre for Biological and Chemical Engineering
Instituto Superior Técnico
Lisbon,Portugal
veronicamartins@ist.utl.pt
Co-Author(s)
Luís Fonseca
Centre for Biological and Chemical Engineering, Lisbon, Portugal
Hugo Ferreira, Daniel Graham, Paulo Freitas
INESC – Microsystems and Nanotechnologies
Joaquim Cabral
Centre for Biological and Chemical Engineering
A Magnetoresistive Biochip for Microbial Analysis of Water Samples
The present work shows the progresses and the applicability of magnetoresistive biochips allied to nanometer-sized superparamagnetic labels
to the detection and quantification of pathogenic microorganisms for water biological quality management. The challenge is to development
a portable device that will be able to carry and perform in situ analysis to detect and quantify almost in real-time, as little as a single unit of
a pathogenic microorganism. The biochip under development is composed of a silicon substrate with integrated magnetoresistive sensors
(spin-valve type) and aluminum current lines. The chip is covered with a 2000 Å silicon dioxide (SiO2) layer, which is used as passivation
layer and a suitable surface for immobilization of bioreceptors. The molecular interaction between probes and labeled-targets, is monitorized
in almost real-time through the variation of the sensor resistance induced by the magnetic moment of the labels located above the sensor.
Salmonella cells were captured by the primary antibody onto chip surface and labeled by a secondary biomolecular recognition using 250 nm
sized amino-functionalized Nanomag beads (Micromod, Germany), which were in-house modified with anti-Salmonella antibody. As control an
anti-E.coli antibody was used as primary antibody to detect non-specific recognitions. An alternative strategy involving hybridization of 23mer
oligonucleotides probes with single stranded complementary DNA sequences bearing a biotin modification in the exposed end, are being
prepared. The biotin will be used for labelling with streptavidin-coated 250 nm Nanomag beads (Micromod, Germany) and further detection
by the sensor. The goal of this work is to validate and combine both strategies in order to achieve a more sensitive and reliable device.
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