LabAutomation 2006 - SLAS

MP49
Thomas Krüger-Sundhaus
University Rostock
Rostock, Germany
thomas.krueger-sundhaus@uni-rostock.de
Where Laboratory Technologies Emerge and Merge
Co-Author(s)
Norbert Stoll, Celisca
Christian Wendler, Celisca
Concept and Design for the Integration of a Complex Laboratory Robot System Into LIMS
At both coordination and execution of individual tasks, at data achieving and surveillance of an automated laboratory system, laboratory
information management systems (LIMS) become ever more important. This includes besides the life visualization of running processes at
the systems also the ability of control software-access. A working laboratory system for chemical applications, installed at the Center for
Life Science Automation (celisca), is managed by a process control system that has been developed in “LabVIEW”. It allows the experiment
planning as well as the visualization and data logging during the run. After firstly being available on the analytical systems’ control PCs, the
row data is read in by the LIMS and stored in a data base. In order to display the data on the user’s web browser the LIMS-server converts
them e.g. into HTML format. For coding data and texts for the exchange via internet as well as for visualization in browsers, XML has
established itself as new standard format. Due to the explicit structuring XML documents can easily be transformed into other formats.
“LabVIEW” supports the bi-directional convergence of XML documents. This allows access to the LIMS from any location in order to
configure the lab robots single-systems or to generate contiguous program sequences.
MP50
Anil Kumar
Institute of Genomics and Integrative Biology
India, Delhi
anilcbt@yahoo.com
Co-Author(s)
Rita Kumar
Purnima Dhall
Institute of Genomics and Integrative Biology
Five days to five minutes: BOD analysis of industrial effluent using biosensor
The conventional BOD test requires 5-7 days, and consequently it is not a suitable method for on-line process monitoring of waste waters.
Thus, it is necessary to develop a method that could circumvent the weaknesses of the conventional method. Present study reports the
determination of BOD within minutes using the developed sensor.
To develop the biosensor for rapid and reproducible BOD estimation of wastewater, different biological and electronic components were
arranged. Initially, change in oxygen concentration was measured and calibrated in terms of electric current covering a range of GGA
concentrations (15 – 60 mg/ml) by putting an electrode immobilized with bacteria. The developed and characterized BOD sensor was used to
analyze the BOD values of different concentrations of the standard GGA solution, the BOD5 of which was simultaneously carried out. A linear
relationship was observed between the current difference and the 5-day BOD of the standard solution up to a concentration of 90 mg l-1.
The lower limit of detection was 1 mg l-1 BOD, by the developed sensor. A good correlation (r = 0.938) was observed between the BOD
values estimated by the conventional method and that by the developed sensor. BOD of a wide range of industrial wastewaters having
low-moderate-high biodegradable wastewater samples could be assessed within a short time period. Developed biosensor is a potable
device and can be used for online monitoring of BOD of wastewaters at industrial site.
127