LabAutomation 2006 - SLAS

MP35
Aoife Gallagher
Deerac Fluidics
Dublin 2, Ireland
aoife@deerac.com
LabAutomation2006
Automated Dispensation of Yttrium Oxide SPA Imaging Beads, Into 1536-Well Plates
Using the Deerac Fluidics’ Equator HTS - Eight Tip Pipetting System
The drive towards miniaturization within the pharmaceutical field has created a need for liquid handling technologies that accurately deliver
low volume reagents to high-density well plates. GE Healthcare’s scintillation proximity assays provide an innovative approach for assay
development and biochemical screening that allows the rapid and sensitive measurement of a wide variety of molecular interactions in a
homogeneous system. Here we demonstrate the successful combination of the use of the Equator HTS eight tip pipetting system to
dispense Streptavidin coupled Yttium Oxide (YOx) imaging beads. 2ul of YOx beads were dispensed into1536-well plates containing an
excess of 3H-biotin. Plates were read on LEADseeker and analyzed for accuracy and reproducibility. CVs of 6-7% were obtained. Outliers
were within spec of 10 or less per 1000 wells. These results demonstrate the efficacy of the Equator HTS with inbuilt stirred reservoir for
the automation of the dispensation of YOx beads to high density plate formats.
MP36
Maojun Gong
University of Cincinnati
Cincinnati, Ohio
gonglemon2@hotmail.com
Co-Author(s)
William R. Heineman
University of Cincinnati
On-Line Sample Preconcentration by Sweeping With Dodecyltrimethylammonium
Bromide in Capillary Zone Electrophoresis
On-line preconcentration of oligonucleotides with a new sweeping carrier was developed by using dodecyltrimethylammonium bromide
(DTAB) below the critical micelle concentration (CMC). The sweeping results with DTAB below and above the CMC were compared. The
DTAB below the CMC benefits the preconcentration of the oligonucleotides, while above the CMC good for hydrophobic small molecules.
The factors affecting sweeping results were optimized and this method was evaluated by constructing calibration curves for thrombin
aptamers. The sweeping scheme produced 112-fold sensitivity enhancement for the oligonucleotides relative to that run in the buffer
without DTAB at the normal polatiry. The sweeping method developed here can be a good reinforcement of the preconcentration scheme
by sweeping when less-hydrophobic analytes are analyzed or the higher separation power of MEKC is unwanted.
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