LabAutomation 2006 - SLAS
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LabAutomation 2006 - SLAS

LabAutomation 2006 - SLAS LabAutomation 2006 - SLAS

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MP31 Frank Doffing IMM - Institut fuer Mikrotechnik Mainz Mainz, Germany doffing@imm-mainz.de LabAutomation2006 Co-Author(s) Dalibor Dadic Klaus Stefan Drese Institut fuer Mikrotechnik Mainz Turning Valves Adapted to Lab-On-A-Chip Applications Enable Directional Flow and Portion out Pre-Defined Volumes The development of preferably simple and simultaneously reliable valve mechanisms is a challenging task by realization of micro-fluidic and lab-on-a-chip systems. Since most applications come along with chemical contamination, commonly polymer-based disposables are required. A highly integrated lab-on-a-chip system requires fluid control and thus active and integrable valves. Metering of certain fluids and subsequent feeding to commonly used channels inside the polymer chip is a further task which can be solved by an appropriate valve mechanism. In the present study we present the design, realization and experimental validation of chip-adapted turning valves which enable both the directional flow as well as the dosage of samples and afterwards feeding into certain channels to allow a following mixing process for instance. Besides a structured disc made from an elastomer and a stiff material compound is adapted on the polymer chip. By turning the cylindrical body the valve works as a directional flow valve. But in addition to this a defined volume, determined by the geometrical dimensions of the metering channel, can be portioned out to a certain channel resp. fluid. The metering channel can be realized on the chip or for smaller volumes on the cylindrical valve body directly. The realized valves are suitable for a wide range of flow rates from 1 µl/min up to 100 ml/min with corresponding pressures ranging from 10 mbar to 1.5 bars. The valves can be actuated by different types of actuators and are successfully applied for lab-on-a-chip systems for sample preparation. MP32 Robert Dunn-Dufault Thermo Electron Burlington, Ontario, Canada rob.dunn-dufault@thermo.com Robert DeWitte Co-Author(s) Marta Kozak Andreas Stelzer Hansjoerg Haas Evaluation of LeadStream’s High Capacity Performance Characteristics in Multiple ADME/Tox Assays A suite of high throughput assays have been implemented on the LeadStream ADME/Tox Solution, and have been shown to produce equivalent results to semi-automated methods that screen for drug-drug interactions, metabolic stability and artificial membrane permeability. In order to assess LeadStream’s performance characteristics, the system was challenged with hundreds of compounds, multiple times, including replicates and standards. Compounds were selected to span a large diversity of chemical structures, with purity above 90%, but with no advance knowledge of how they would behave in each of the assays. In parallel, the same compounds were analyzed with equivalent semi-automated methods. This poster reports performance characteristics of LeadStream, including throughput, turn-around time, as well as measures of technical robustness, accuracy, precision and ease-of-use. 118

MP33 Joshua Edel Harvard University Rowland Institute Cambridge, Massachusetts edel@rowland.harvard.edu Where Laboratory Technologies Emerge and Merge Co-Author Amit Meller Harvard University Probing Single Molecule Dynamics on the Nanoscale In this talk an approach will be described discussing the development and application of using confined fluidic systems to probe single molecule dynamics of DNA oligomers using fluorescence lifetime resonance energy transfer and confocal spectroscopy. The utilization of fluidic channels allows us to precisely control the conditions of a local environment. Micro and nanochannels have recently become popular in applications such as nucleic acid separations, DNA sequencing, and cell manipulations. The benefits of downsizing include enhanced analytical performance, reduced separation and analysis times, reduced reaction times, smaller sample sizes, reduced reagent waste, high levels of functional integration and automation, and reduced instrument footprints when compared to conventional (larger) analogues. In the work that will be discussed, we utilize the added benefits of downsizing to monitor DNA bubble formation, DNA – DNA interactions, as well as DNA-fluorophore interactions in real time at the single molecule level. The ability to measure biological activity of individual biomolecules by single-molecule fluorescence methods can be limited by the nonideal properties of the fluorophores. As will be described, the source of these phenomena is related to uncontrolled changes in the immediate environment of the fluorophore. MP34 Richard Ellson Labcyte Sunnyvale, California ellson@labcyte.com Co-Author(s) Mitchell Mutz, Labcyte Inc. David Harris Debunking the Myth – Using Fluorescein in Analytical Measurements of Fluid Transfers Under certain conditions, fluorescein will photo-bleach. For example, in laser-based confocal microscopy of fluorescein-conjugated antibodies in cells, this photo-bleaching can limit the utility of fluorescein and force the use of more expensive dyes. However, fluorescence measurements of nanoliter volumes of DMSO containing fluorescein reveal excellent accuracy and precision. We show that fluorescein works extremely well in these analyses with no measurable depletion due to photo-bleaching. We further show that previously reported examples of photo-bleaching in this application may be due to reader drift and not the destruction of fluorescein. Fluorescein costs approximately $34 for 100 g. Photo-stable compounds cost significantly more with price per gram ranging from 10,000 to 200,000 times greater. 119

MP33<br />

Joshua Edel<br />

Harvard University<br />

Rowland Institute<br />

Cambridge, Massachusetts<br />

edel@rowland.harvard.edu<br />

Where Laboratory Technologies Emerge and Merge<br />

Co-Author<br />

Amit Meller<br />

Harvard University<br />

Probing Single Molecule Dynamics on the Nanoscale<br />

In this talk an approach will be described discussing the development and application of using confined fluidic systems to probe single<br />

molecule dynamics of DNA oligomers using fluorescence lifetime resonance energy transfer and confocal spectroscopy. The utilization of<br />

fluidic channels allows us to precisely control the conditions of a local environment. Micro and nanochannels have recently become popular<br />

in applications such as nucleic acid separations, DNA sequencing, and cell manipulations. The benefits of downsizing include enhanced<br />

analytical performance, reduced separation and analysis times, reduced reaction times, smaller sample sizes, reduced reagent waste, high<br />

levels of functional integration and automation, and reduced instrument footprints when compared to conventional (larger) analogues. In the<br />

work that will be discussed, we utilize the added benefits of downsizing to monitor DNA bubble formation, DNA – DNA interactions, as well<br />

as DNA-fluorophore interactions in real time at the single molecule level. The ability to measure biological activity of individual biomolecules<br />

by single-molecule fluorescence methods can be limited by the nonideal properties of the fluorophores. As will be described, the source of<br />

these phenomena is related to uncontrolled changes in the immediate environment of the fluorophore.<br />

MP34<br />

Richard Ellson<br />

Labcyte<br />

Sunnyvale, California<br />

ellson@labcyte.com<br />

Co-Author(s)<br />

Mitchell Mutz, Labcyte Inc.<br />

David Harris<br />

Debunking the Myth – Using Fluorescein in Analytical Measurements of Fluid Transfers<br />

Under certain conditions, fluorescein will photo-bleach. For example, in laser-based confocal microscopy of fluorescein-conjugated<br />

antibodies in cells, this photo-bleaching can limit the utility of fluorescein and force the use of more expensive dyes. However, fluorescence<br />

measurements of nanoliter volumes of DMSO containing fluorescein reveal excellent accuracy and precision. We show that fluorescein works<br />

extremely well in these analyses with no measurable depletion due to photo-bleaching. We further show that previously reported examples of<br />

photo-bleaching in this application may be due to reader drift and not the destruction of fluorescein. Fluorescein costs approximately $34 for<br />

100 g. Photo-stable compounds cost significantly more with price per gram ranging from 10,000 to 200,000 times greater.<br />

119

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