LabAutomation 2006 - SLAS
LabAutomation 2006 - SLAS
LabAutomation 2006 - SLAS
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MP31<br />
Frank Doffing<br />
IMM - Institut fuer Mikrotechnik Mainz<br />
Mainz, Germany<br />
doffing@imm-mainz.de<br />
<strong>LabAutomation</strong><strong>2006</strong><br />
Co-Author(s)<br />
Dalibor Dadic<br />
Klaus Stefan Drese<br />
Institut fuer Mikrotechnik Mainz<br />
Turning Valves Adapted to Lab-On-A-Chip Applications Enable Directional Flow and<br />
Portion out Pre-Defined Volumes<br />
The development of preferably simple and simultaneously reliable valve mechanisms is a challenging task by realization of micro-fluidic<br />
and lab-on-a-chip systems. Since most applications come along with chemical contamination, commonly polymer-based disposables<br />
are required. A highly integrated lab-on-a-chip system requires fluid control and thus active and integrable valves. Metering of certain fluids<br />
and subsequent feeding to commonly used channels inside the polymer chip is a further task which can be solved by an appropriate<br />
valve mechanism.<br />
In the present study we present the design, realization and experimental validation of chip-adapted turning valves which enable both the<br />
directional flow as well as the dosage of samples and afterwards feeding into certain channels to allow a following mixing process for<br />
instance. Besides a structured disc made from an elastomer and a stiff material compound is adapted on the polymer chip. By turning<br />
the cylindrical body the valve works as a directional flow valve. But in addition to this a defined volume, determined by the geometrical<br />
dimensions of the metering channel, can be portioned out to a certain channel resp. fluid. The metering channel can be realized on the chip<br />
or for smaller volumes on the cylindrical valve body directly.<br />
The realized valves are suitable for a wide range of flow rates from 1 µl/min up to 100 ml/min with corresponding pressures ranging from<br />
10 mbar to 1.5 bars. The valves can be actuated by different types of actuators and are successfully applied for lab-on-a-chip systems for<br />
sample preparation.<br />
MP32<br />
Robert Dunn-Dufault<br />
Thermo Electron<br />
Burlington, Ontario, Canada<br />
rob.dunn-dufault@thermo.com Robert DeWitte<br />
Co-Author(s)<br />
Marta Kozak<br />
Andreas Stelzer<br />
Hansjoerg Haas<br />
Evaluation of LeadStream’s High Capacity Performance Characteristics in Multiple<br />
ADME/Tox Assays<br />
A suite of high throughput assays have been implemented on the LeadStream ADME/Tox Solution, and have been shown to produce<br />
equivalent results to semi-automated methods that screen for drug-drug interactions, metabolic stability and artificial membrane<br />
permeability. In order to assess LeadStream’s performance characteristics, the system was challenged with hundreds of compounds,<br />
multiple times, including replicates and standards. Compounds were selected to span a large diversity of chemical structures, with purity<br />
above 90%, but with no advance knowledge of how they would behave in each of the assays. In parallel, the same compounds were<br />
analyzed with equivalent semi-automated methods. This poster reports performance characteristics of LeadStream, including throughput,<br />
turn-around time, as well as measures of technical robustness, accuracy, precision and ease-of-use.<br />
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