LabAutomation 2006 - SLAS

MP23
Robin Clark
deCODE Biostructures
Bainbridge Island, Washington
rclark@decode.com
LabAutomation2006
Co-Author(s)
Alexandrina Muntianu
Hans-Thomas Richter
Denise Conner
Lawrence Chun
Alex Burgin
Lance Stewart
DeCODE BioStructures
Protein Maker: an Automated System for Protein Purification
The Protein Maker is an automated system for parallel liquid chromatography at medium scale (1-50 mg of protein). Protein solutions,
wash buffers and elution buffers are delivered under positive pressure to up to 24 columns by automated syringe pumps. The 24 syringe
pumps are connected to 8-way valves, with each pump and valve controlled independently through the software. A gantry with XYZ
directional control carries two 24-port manifolds: one for sample loading and one for column outlets. Flow rates adjustable from 0.25 to
1000 ml/min. Column fractions are delivered into 24-well block plates at any of 20 deck locations. Protein Maker can be used to purify up
to 24 different proteins in parallel on duplicate columns, simultaneously test multiple purification strategies on one or a few proteins or carry
out purification schemes on multiple columns in tandem. New developments in progress include: 1) fully automated purification of up to 8
proteins on 3 different columns in succession, 2) UV and conductivity monitoring, 3) provision for load and elution volumes up to 500 ml, 4)
a relational database for operations and parameters. Application results and software interface improvements will be presented.
MP24
Wendell Coltro
University of São Paulo
São Carlos-SP, Brazil
wendell@iqsc.usp.br
Co-Author(s)
Wendell Karlos
Tomazelli Coltro
University of Sao Paulo
José Alberto, Fracassi da Silva, State University of Campinas
Emanuel Carrilho, University of Sao Paulo
Disposable Electrophoresis Microchips With Integrated Electrodes for Capacitively
Coupled Contactless Conductivity Detection
We describe the development of electrophoresis microchips with integrated electrodes on the polyester films for capacitively coupled
contactless conductivity detection (C4D). The top polyester substrate contained the two electrodes (Ti/Pt-200 nm) for C4D, which were
formed by photolithographic lift off process. The microchannels network was fabricated by a direct-printing process in the bottom polyester
substrate. The layout of the device was drawn using CorelDraw 11.0 software and it was printed on polyester films out by LaserJet printer.
The toner layer deposited (6 micrometers) by the laser printer defines the depth of the microchannel. The access in the channels was made
by drilling a hole using an adapted paper driller. The perforated cover and the base were aligned and laminated together, producing the
single toner layer structures with access holes. In the lamination step the electrodes had been placed outside of the channel, i.e., isolated
by the thickness of the polyester film itself (100 micrometers). A mixture of potassium, sodium and lithium ions was used to evaluate the
separation performance of the integrated C4D. The running buffer was 20 mmol L-1 2-(N-morpholino)ethanesulfonic acid/histidine at pH
6.0. The sample plug (270 pL), containing 50 umolL-1 of each cation, was separated in approximately 60 sec and detected at 400kHz and
10 Vpp. The proposed microdevice presented satisfactory results in terms of repeatability, sensitivity, separation efficiency and resolution.
To our knowledge these disposable microchips with integrated electrodes have the lowest cost/device and can be extensively used in any
applications in-the-field.
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