LabAutomation 2006 - SLAS
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LabAutomation 2006 - SLAS

LabAutomation 2006 - SLAS LabAutomation 2006 - SLAS

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MP07 Varouj Amirkhanian eGene, Inc. Irvine, California vamirkhanian@egeneinc.com Automated High-Speed Genetic Analyzer LabAutomation2006 Co-Author Ming-Sun Liu, eGene, Inc. We present a bench-type, high-performance and high-speed genetic analyzer, that uses cost-effective parallel multi-channel gel-capillary electrophoresis system with novel (patented) fluorescence type detection for bio-molecules analysis.12-DNA samples are automatically injected and analyzed simultaneously using a multiple usage and disposable multi-capillary (12-Channel) gel-cartridges. Using commercially available dsDNA size markers (i.e. FX174 DNA–Hae III digest dsDNA fragments) as indicators, the system provides high resolving power (

MP09 Alex Batchelor Cambrex Bio Science Nottingham Nottingham, United Kingdom alex.batchelor@cambrex.com Where Laboratory Technologies Emerge and Merge Co-Author(s) Lee Walker Anthony Pitt PDELight - A Novel, Generic and Simple High Throughput Assay for Screening cAMP-Dependent Phosphodiesterases In the cell phosphodiesterases (PDEs) function in conjunction with adenylate cyclases to regulate the amplitude of the ubiquitous 2nd messenger signalling molecule, cyclic adenosine monophosphate (cAMP). PDEs catalyze the hydrolysis of cAMP to adenosine monophosphate (AMP). There are at least 11 different families of PDEs most of which contain more than one isozyme. Their substrate specificities, kinetics and tissue specific expression make PDEs drugable targets for a range of diseases. A number of HTS assays are used to identify inhibitors of cAMP-PDEs. However these are either rad-based, require the use of beads, modified substrate or antibodies and are time consuming to perform. We introduce a novel luminescent HTS assay which offers a simple alternative to the current cAMP-PDE assays. The AMP produced from PDE hydrolysis of cAMP is quantified using a robust and highly sensitive luciferase-based luminescent reagent. The AMP is directly converted to ATP and quantified as light. Nearly a photon of light is emitted for every molecule of AMP produced. The assay is extremely simple to use and can be run in a number of ways to suit the user. Data represented in this study demonstrates the principle and performance of the assay. MP10 Michael Benedetti Buck Institute Novato, California mbenedetti@buckinstitute.org Co-Author(s) Matthew Gill Anders Olsen Amanda Foster Gordon Lithgow Development of High-Throughput Screens for Anti-Aging Compounds in the Nematode Caenorhabditis Elegans The nematode Caenorhabditis elegans provides an excellent model organism for investigating the aging process. These worms have a short lifespan of approximately 20 days and many single gene mutations have been found that more than double their lifespan. There is now considerable interest in identifying drugs that can influence nematode lifespan as they may provide novel therapeutic targets for amelioration of age related disease. Small scale, targeted screens have demonstrated that nematode lifespan can be increased by treatment with drugs such as anti-oxidants and anticonvulsants. It is likely that the development of high throughput screening methods will facilitate the discovery of many more compounds that are able to extend the lifespan of C. elegans. C. elegans is well suited to HTS as it is easy to grow large isogenic populations and maintain worms in multi-well plates. The use of an intact organism for the screening process also has many advantages over cell based assays. Most interventions that increase lifespan also increase resistance to acute stress. This makes it possible to assess the effect of a candidate compound within a few days using stress resistance assays as a surrogate for lifespan. Here we present different in vivo automated screens for compounds that affect survival following a stress. We discuss the results of small 2000 compound screens and show that the screens are adaptable to HTS. We discuss the hurdles to automating whole organism screens and their possible solutions. 107

MP07<br />

Varouj Amirkhanian<br />

eGene, Inc.<br />

Irvine, California<br />

vamirkhanian@egeneinc.com<br />

Automated High-Speed Genetic Analyzer<br />

<strong>LabAutomation</strong><strong>2006</strong><br />

Co-Author<br />

Ming-Sun Liu, eGene, Inc.<br />

We present a bench-type, high-performance and high-speed genetic analyzer, that uses cost-effective parallel multi-channel gel-capillary<br />

electrophoresis system with novel (patented) fluorescence type detection for bio-molecules analysis.12-DNA samples are automatically<br />

injected and analyzed simultaneously using a multiple usage and disposable multi-capillary (12-Channel) gel-cartridges. Using commercially<br />

available dsDNA size markers (i.e. FX174 DNA–Hae III digest dsDNA fragments) as indicators, the system provides high resolving power<br />

(

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