LabAutomation 2006 - SLAS

MP05
Dave Smith
TTP LabTech
Royston, Hertfordshire, United Kingdom
dave.smith@ttplabtech.com
Where Laboratory Technologies Emerge and Merge
Co-Author(s)
Yan Wang
Robert Davis
Kalypsys Inc.
Wayne Bowen
TTP LabTech Ltd
An Ultra-High Throughput Approach to High Content Screening in 1536-Well Format
Kalypsys’ technology has enabled unprecedented levels of efficiency and economy in primary screening, and has proved especially useful
at rapidly profiling screening hits in selectivity and safety assays. Combining throughput of over 1 million assay wells per day with on-line
storage capacity of over 2 million compounds, the Kalypsys System provides unmatched screening efficiency. To effectively utilize whole
cell assay formats in primary and secondary screening, image-based high content readers require multi-channel capability, rapid read
times and compatibility with high-density plate formats. The Acumen Explorer offers whole-well, high content analysis of 1536 microplates
in less than 10 minutes per plate, while collecting data for up to four colours in multiplex protocols, thus combining the object-recognition
capabilities of image-based systems with short read times. Here, we demonstrate the powerful integration of an Acumen Explorer with
Kalypsys technology, with capability to screen > 300,000 wells per day of high content data.
MP06
Arne Allwardt
University of Rostock
Rostock, Germany
arne.allwardt@celisca.de
The HPMR 50-96 advance - Always a Step Ahead
Co-Author(s)
Silke Holzmüller-Laue, Celisca
Kerstin Thurow, University Rostock
The further reduction of the product development time, increasing prices for basic materials and limited laboratory capacities increase the
requirements to the assigned technologies and require particulary multivariate laboratory systems. The devices used in these systems
are characterized by an increase of the reactions per time unit and the reduction of the reaction volumes with consideration of compact
dimensions. They have to be variable integrable into usual laboratory robot systems in hard and software.
The multi-parallel high pressure reactor HPMR 50-96 fulfills these requirements. It ensures the contemporaneous execution of 96 reactions
in a glass microplate under reaction pressures and temperatures of 50 bar and 100°C. The mixing of the reagents is realized magnetically
with small stirdiscs in every well. A local control system can be used to control the reactor. The integration into a laboratory robot system or
the connection to a LIMS is likewise possible.
The HPMR 50-96 is now available in a first advanced version with an improved tempering and a new gas management system. The number
and power of the integrated peltier elements has been increased for an improved tempering. Together with an improved recooling of the
elements the heating and cooling times of the pressure tank and the reagents could be dramatically reduced. The new gas management
system permits a flushing with inert gas even with a closed pressure tank and thus the safe exchange of the tank atmosphere with inert
gas is possible.
105