POSTERS - BLAST X - University of Utah
POSTERS - BLAST X - University of Utah
POSTERS - BLAST X - University of Utah
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<strong>BLAST</strong> X Poster #36<br />
CrdC NEGATIVELY REGULATES CheW3 AND CheA3 INTERACTION DURING SIGNAL<br />
TRANSDUCTION IN MYXOCOCCUS XANTHUS<br />
Jonathan Willett, Susanne Mueller, John Kirby<br />
<strong>University</strong> <strong>of</strong> Iowa, Department <strong>of</strong> Microbiology, 3-403 Bowen Science Building<br />
51 Newton Road, Iowa City, IA 52242<br />
Previous work on the Che3 system <strong>of</strong> Myxococcus xanthus has led to the discovery that<br />
a chemosensory signal-transduction system affects developmentally regulated gene expression.<br />
The Che3 system contains homologs <strong>of</strong> the prototypical chemotaxis proteins such as CheA,<br />
CheW, CheB, CheR, and MCPs but lacks a CheY homolog. The output <strong>of</strong> the system consists<br />
<strong>of</strong> a NtrC transcriptional activator termed CrdA. There are several other unique proteins<br />
comprising the Che3 pathway besides CrdA, with one <strong>of</strong> the more interesting being a protein<br />
termed CrdC. CrdC is contained in the same transcriptional unit as CheW3. Preliminary data<br />
has shown that CrdC interacts strongly CheW3 in yeast-two hybrid experiments. More<br />
interestingly, the presence <strong>of</strong> CrdC in a yeast-three hybrid experiment has been shown to inhibit<br />
the interaction between CheA3 and CheW3. We hypothesize that CrdC thereby presents a<br />
unique mechanism for regulation <strong>of</strong> signal transduction through the Che3 pathway by<br />
decoupling CheA3 and CheW3.<br />
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