POSTERS - BLAST X - University of Utah
POSTERS - BLAST X - University of Utah
POSTERS - BLAST X - University of Utah
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
<strong>BLAST</strong> X Wed. Morning Session<br />
STRUCTURE AND FUNCTION OF THE HELICOBACTER PYLORI CHEMORECEPTOR TlpB<br />
John Goers, Nathan Henderson, S. James Remington, Karen Ottemann * and Karen Guillemin<br />
Institute <strong>of</strong> Molecular Biology, <strong>University</strong> <strong>of</strong> Oregon, Eugene OR, 97403<br />
* Environmental Toxicology, UC Santa Cruz, Santa Cruz, CA 95064<br />
We have shown that the Helicobacter pylori chemoreceptor TlpB is required for<br />
chemotaxis away from acid and from the quorum-sensing molecule autoinducer-2. Here we<br />
report the determination <strong>of</strong> an atomic resolution crystal structure for the periplasmic domain <strong>of</strong><br />
TlpB. The structure reveals a PAS domain inserted into a pair <strong>of</strong> antiparallel helices and shows<br />
unexpected structural homology to the LuxQ receptor from Vibrio harveyi. Furthermore, the PAS<br />
domain tightly binds urea, suggesting that the TlpB receptor may be associated with detection <strong>of</strong><br />
urea. We present mutational and physical evidence for interaction <strong>of</strong> TlpB with urea and its role<br />
in chemotaxis.<br />
30
Change language