POSTERS - BLAST X - University of Utah
POSTERS - BLAST X - University of Utah
POSTERS - BLAST X - University of Utah
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<strong>BLAST</strong> X Poster #65<br />
IN VIVO STUDY OF THE TWO-COMPONENT SIGNALLING NETWORK IN E. COLI<br />
Erik Sommer and Victor Sourjik<br />
Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), Im Neuenheimer Feld 282,<br />
69120 Heidelberg, Germany<br />
Contact: e.sommer@zmbh.uni-heidelberg.de<br />
Two-component systems are the most widespread sensing systems in prokaryotes and<br />
lower eukaryotes, with multiple members <strong>of</strong> this class being present in one organism. We are<br />
interested in investigating the interconnection among different two-component signalling<br />
pathways in Escherichia coli. To map interactions between the pathways in vivo and to study<br />
relative cellular distribution <strong>of</strong> their proteins, we assay real-time dynamics <strong>of</strong> protein interactions<br />
and their dependencies on stimulation using fluorescence imaging and fluorescence resonance<br />
energy transfer (FRET)- and fluorescence recovery after photobleaching (FRAP)-microscopy.<br />
Additionally, intracellular processing <strong>of</strong> sensed stimuli with regard to amplification, integration<br />
and possible cross-talk between the systems will be investigated. Such analysis will help to<br />
establish an integral picture <strong>of</strong> cell signalling performed by prokaryotic organisms.<br />
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