POSTERS - BLAST X - University of Utah
POSTERS - BLAST X - University of Utah
POSTERS - BLAST X - University of Utah
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<strong>BLAST</strong> X Poster #60<br />
LOCALIZATION OF THE CHEMOTAXIS PROTEINS IN BACILLUS SUBTILIS<br />
Kang Wu and Christopher Rao<br />
<strong>University</strong> <strong>of</strong> Illinois at Urbana-Champaign<br />
The Bacillus subtilis chemotaxis pathway utilizes three proteins – CheC, CheD, and<br />
CheV - not found in Escherichia coli. These proteins are thought to be involved in two<br />
methylation-independent adaptation systems not found in E. coli. While the functions <strong>of</strong> these<br />
proteins have been characterized to some degree, the details concerning their interactions<br />
within the polar signaling complex are still unknown. To better understand these interactions, we<br />
are investigating the factors influencing the localization <strong>of</strong> the B. subtilis chemotaxis proteins<br />
using immun<strong>of</strong>luorescence and fluorescent protein fusions. Of significance, we have<br />
constructed functional fluorescent protein fusions to CheC and CheD and also explored their colocalization<br />
using two-color immun<strong>of</strong>luorescence. Using these approaches, we have quantified<br />
the localization <strong>of</strong> these proteins in a number <strong>of</strong> different mutants. This poster will discuss some<br />
<strong>of</strong> our preliminary results regarding the factors influencing localization <strong>of</strong> the chemotaxis<br />
proteins in B. subtilis.<br />
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