POSTERS - BLAST X - University of Utah
POSTERS - BLAST X - University of Utah
POSTERS - BLAST X - University of Utah
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<strong>BLAST</strong> X Poster #56<br />
REACTIVE ALDEHYDES AND MOTILITY IN ESCHERICHIA COLI K12<br />
Changhan Lee, Junghoon Lee, Jongchul Shin, Insook Kim, Kwanghee Baek, and Chankyu<br />
Park<br />
Department <strong>of</strong> Biological Sciences, KAIST, Daejon, Korea<br />
Graduate School <strong>of</strong> Biotechnology, Kyung Hee <strong>University</strong>, Yongin, Korea<br />
The short chain carbohydrates such as glyoxal and methylglyoxal are generated in vivo<br />
from various sugars either by an oxidative stress or by a cellular metabolism, which are believed<br />
to be removed by the glutathione-dependent glyoxalase system. We isolated a number <strong>of</strong> E. coli<br />
mutants conferring resistance or sensitivity to these aldehydes and found that some <strong>of</strong> them<br />
affect motility. In the case <strong>of</strong> glyoxalse I mutant (gloA) that is deficient in the removal <strong>of</strong><br />
aldehyde using glutathione, significant reductions in the free-swimming as well as in swarming<br />
behaviors were observed. When we introduced the flhDC-lacZ fusion contained in phage<br />
Lambda into the wild-type and gloA strains, expression <strong>of</strong> LacZ was considerably reduced in the<br />
gloA mutant compared to that <strong>of</strong> wild type, suggesting that a decrease in flagellar expression is<br />
responsible for the impaired motility. This is further confirmed by the reduced expression <strong>of</strong><br />
flagellin as detected by anti-flagellin antiserum. We observed similar effects <strong>of</strong> other glyoxalrelated<br />
genes on motility, suggesting a possibility that an intracellular level <strong>of</strong> aldehyde is<br />
somehow associated with flagellar gene expression. Cellular redox change due to an aldehyde,<br />
affecting motility, will be discussed.<br />
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