BioSciences - Polysciences, Inc.

88
Microbiology
BioSciences
Gram’s Stain Kit with Iodine .................................................
Gram stain permits the separation of all bacterial species into two large groups, those which retain
the primary dye (gram-positive) and those which lose the primary dye and take the color of the
secondary dye (gram-negative). The mechanism of gram stain is based on the distinctive chemistry
and physical properties of the cell wall, possibly the lipid content. However, the exact mechanism of
gram stain is still unknown. Technical Data Sheet #749
Kit contains: Crystal Violet, Gram’s Iodine, Decolorizer and Safranin (8 oz. bottle of each).
Gram’s Stain Kit with Stabilized Iodine ........................................
The gram stain permits the separation of all bacterial species into two large groups, those which
retain the primary dye (gram-positive), and those which lose the primary dye and take the color of
the secondary dye (gram-negative). The mechanism of gram stain is based on the distinctive chemistry
and physical properties of the cell wall, possibly the lipid content. However, the exact mechanism
of gram stain is still unknown.
AFB Ziehl-Neelson Kit (Hot Method) CH6g .....................................
Bacteriological stain kit used to identify acid-fast organisms, mainly Mycobacteria.
Helpful in diagnosing Mycobacterium tuberculosis since its lipid rich cell wall
makes it resistant to Gram stain. Can also be used to stain other bacteria like
Nocardia. Kit contains a primary dye (carbol-fuchsin), a decolorizer (acid-alcohol)
and a counter stain (methylene blue). Decolorizer is used to decolorize the bacte-
ria that “dislike” the primary stain. Counter stain is used to stain those bacteria
which were decolorized by the acid alcohol. Acid-fast bacilli will stain bright red,
while non-acid fast organisms will stain blue or green. Technical Data Sheet #746
Kit contains: Carbol Fuchsin (Ziehl-Neelson), Acid Alcohol and Methylene Blue
(8 oz. bottle of each).
Szechrome NAS U3g ........................................................
Suitable for the determination of the nitrate content of natural waters, industrial waste
effluents, soil, plant and meat extracts, tinned goods, biological fluids (sputum, urine),
chemicals, fertilizers and drugs. NAS has been used in place of the phenoldisulfonic
acid, brucine and chromotropic acid methods used previously. This sensitive reagent
reacts rapidly to produce stable colors suitable for precise quantitative determinations
or rapid estimations in routine field work. Szechrome NAS produces color reactions
which are specific and proportional to the nitrate content of the sample tested. Optical
density (OD) at 570nm after 5 min. development is proportional to nitrate in the
range of 2-20ppm. Technical Data Sheet #239
Microloop ® Calibration Gauge
Microloop ® Calibration Gauge for 1µl .........................................
Description: Double ended tool. Each end has an accurately machined
solid cylinder of stainless steel of defined diameter. Correctly calibrated
loop will fit over green end, but not red end.
Features: Ensure accuracy of procedures, simple to use, robust construction
ensures many years of use.
Microring ® Bacterial Detectors
Microring ® AC .............................................................
Paper ring with 3 individual printed tips containing various antimicrobial
reagents. The resistance or sensitivity patterns can be used to help identify
anaerobic cocci. (50 rings) Technical Data Sheet #741
Features: Ready to use, more convenient than individual discs, 12 month shelf life.
For more information please call (800) 523-2575 or visit: polysciences.com
Mycobacterium tuberculosis
bacteria revealed using acidfast
Ziehl- Neelson stain; Magnified
1000X. Image courtesy
of CDC Publich Health Library/
Dr. George P. Kubica.
Catalog # Size
24668-1 1 kit
24667-1 1 kit
24669-1 1 kit
08762-5 5 g
24652-1 1 gauge
24654-1 50 rings