BioSciences - Polysciences, Inc.

Microbiology
BioSciences
Plastic Microloops ® 5µl - individually packed ...................................
Sterile disposable plastic 5µl inoculating loops for precise volumetric sampling
and transfer of material for accurate analysis. Individually packed in peel pouch.
Accurate sampling, Consistent and reproducible results, Precision moulded – no
gouging of media, no need for Bunsen burner, no aerosols, color coded for size.
Dyes and Stains
Crystal Violet ..............................................................
Used in the Gram’s Staining method for the difference of Gram Positive and Gram Negative bacteria.
Gram’s Decolorizer (50/50)
Used in Gram Staining protocols. The Gram stain is used to classify bacteria on the basis of their
forms, sizes, cellular morphologies, and Gram reactions.
Catalog # Size
Gram’s Decolorizer (50/50) 24683-1 1 gal
Gram’s Decolorizer (75/25) 24682-1 1 gal
Gram’s Iodine - Stabilized ...................................................
A component of the Gram Stain. Gram stain is used to classify bacteria on the basis of their forms,
sizes, cellular morphologies and Gram reactions.
Stain Kits
AFB Kinyoun Kit (Cold Method) CHVWX7g .....................................
Method of staining acid-fast microorganisms, specifically mycobacterium. Procedure
is similar to Ziehl-Neelson stain, but does not involve heating the slides.
Kinyoun staining method uses carbol-fuchsin as a primary stain, followed by
decolorization with an acid-alcohol solution and methylene blue as a counterstain.
Kinyoun carbol-fuschsin has a greater concentration of phenol and basic
fuchsin and does not require heating in order to stain properly. When viewed
under a microscope, Kinyoun stained slides will show acid-fast organisms as red
and non acid-fast organisms as blue. Technical Data Sheet #747
Kit contains: Carbol Fuchsin (Kinyoun), Acid Alcohol and Methylene Blue
(8 oz. bottle of each).
AFB Rhodamine-Auramine Kit (Fluorescent) ...................................
The identification of Mycobacteria with rhodamine-auramine is due to the affinity of the mycolic
acid in the cell walls for the fluorochromes. Dyes will bind to the Mycobacteria, which appear bright
yellow or orange against a greenish background. The potassium permanganate helps prevent nonspecific
fluorescence. All acid-fast organisms will be stained by rhodamine-auramine, including the
sporozoan parasites. Slides stained with rhodamine-auramine may be restained with Ziehl-Neelsen
or Kinyoun stain directly, as long as the oil has been removed. This provides a convenient method of
confirming and differentiating morphology of positive slides with the traditional stains.
Technical Data Sheet #748
Kit contains: Rhodamine - Auramine, Fluorescent Decolorizer, Potassium - Permanganate
(8 oz. bottle of each).
24650-1 1000
loops
24686-1 1 gal
Gram’s Iodine ..............................................................
Used in Gram Staining protocols. The Gram stain is used to classify bacteria on the basis of their forms,
sizes, cellular morphologies and Gram reactions.
24684-1 1 gal
0.4% Safranin, Ready-to-Use CH4g ...........................................
0.4% Safranin is the classic counterstain in a Gram stain. Our ready-to-use format
eliminates the exposure to potentially irritating powdered dyes.
24672-1 1 gal
Francisella tularensis using a
Safranin stain, Magnified 1000X.
Courtesy of CDC Public Health
Library / Dr. P. B. Smith.
Direct fecal smear is stained to
detect Cryptosporidium sp., an
intracellular protozoan parasite
using a modified cold Kinyoun
acid-fast staining technique.
Image courtesy of CDC Public
Health Library
24685-1 1 gal
24670-1 1 kit
24666-1 1 kit
For more information please call (800) 523-2575 or visit: polysciences.com 87