BioSciences - Polysciences, Inc.

84
Fluorescent Dyes & Stains
BioSciences
Fungi-White ...............................................................
Suitable to use in identification of fluorescent fungal elements and trichomonas detection. Rapid
chemo-fluorescent method useful for the detection of Microsporidia and Acanthamoeba spp. The
Fungi-White dye fluoresces when exposed to ultraviolet light (below 400nm wavelength). The
procedure is useful on fecal smears, cell cultures, or other sample materials. To use this method
on plastic sections requires modifications to the standard protocol. Technical Data Sheet #756
Kit Contains: 6 x 5ml Fungi-White Reagent A, 6 x 5ml Stain Reagent B
B. Mori & L. M. Bellani Biotech. Histochem., 1996, 71:71-72
Hanker-Yates reagent HVWX7d ...............................................
A specific, sensitive and non-carcinogenic chromogen for immunoperoxidase techniques.
Contains p-phenylenediamine and pyrocatechol. Gives a blue reaction product.
Technical Data Sheet #204
Histochem. J., 9, 789 (1977); Am. J. Clin. Path., 75, 367 (1981)
Hydroethidine (Dihydroethidium bromide) [104821-25-2] HU5cd .................
MW 315.5 mp 202-206 ° Exc. max: 365nm Emiss. max: 420nm
Reduced ethidium bromide. A vital stain. Enters and stains living cells without
cellular trauma. Double staining system. Stains cytoplasm blue and chromatin
red. Excellent cellular retention. Remains incorporated in chromatin with virtually
no leakage. Essentially non-toxic. Shows no toxicity at levels useful for
visualizing chromatin. Technical Data Sheet #351 Biotechnology, 3, 4 (1985)
Lissamine Rhodamine B sulfonyl chloride, ~99% [62796-29-6] H3g ................
MW 577.1
Fluorescent protein stain with bright orange fluorescence. Used to visualize actin filaments in living
amoebas. Useful for waste water testing and various microbiological applications.
J. Cell. Biol. 86, 590 (1980)
Mithramycin [18378-89-7] VWX7f .............................................
(Plicamycin; Aureolic acid; Mithracin) MW 1085.18 mp 180-183°
Fluorescent DNA dye.
Stain Technol., 60, 145 (1985); Merck Index 11, 7510
Naphthalene-2,3-dicarboxaldehyde [7149-49-7] HY4g ...........................
Highly Purified Grade-single spot TLC Fluorogenic derivatizing agent for amines. Useful for fluorescent
determination of serum arginine and for trace amino acid and peptide analysis.
Nitroblue tetrazolium chloride (NBT) [298-83-9] HU6ae ..........................
MW 817.65 mp 215-217°
Used for estimating dehydrogenases and other oxidases. Also used in the detection
of nucleic acid hybridization and in the detection of ascorbate peroxidase activity
in native gels. Totally soluble and formazan free. Methods Enzymol., 6, 958 (1963); Anal.
Biochem., 56, 353 (1973); 212, 540 (1993), J. Clin. Lab. Anal., 7, 174 (1993)
Osmium ammine-B [48016-91-7] U5g ..........................................
Stable DNA stain.
J. Histochem. Cytochem., 37, 395 (1989)
Pneumocystis carinii control slides U2g .......................................
Designed to be used with our Fungi Fluor Kit Pneumocystis Kit (Cat. #22363),
these slides are unstained. 10 slides per box. Technical Data Sheet #483
Propidium iodide [25535-16-4] HVWX6g .......................................
MW 668.41 mp 220.225º
Fluorescent marker. Used as a nuclear counterstain and for In situ Hybridization (ISH).
J. Histochem. Cytochem., 35, 123 (1987)
For more information please call (800) 523-2575 or visit: polysciences.com
Catalog # Size
24692-1 1 kit
08661-5 5x1 g
17084-50 50 mg
08074-100 100 mg
09330-5 5 mg
21486-100 100 mg
00928-500 500 mg
00928-1 1 g
21033-100 100 mg
22251-1 1 box
03748-100 100 mg