BioSciences - Polysciences, Inc.

Fluorescent Dyes & Stains
Fluorescence Microscopy
BioSciences
Acridine orange, C.I. 46005, very high purity [65-61-2] HU5e .....................
(3,6-Bis[dimethylamino]acridine hydrochloride hydrate) MW 301.83
DNA intercalating dye. A grade of acridine orange of exceptionally high purity, suitable for
quantitative work. Free of inorganic salts. A specific stain for RNA, used as a 2% solution containing
1% lanthanum acetate in 15% acetic acid.
J. Histochem. Cytochem., 22, 495 (1974); 31, 737 (1983); 44, 393 (1996); 49, 921 (2001); J. Lab Med., 15(3), 180 (1984)
Acridine mutagen ICR 191 [17070-45-0] HU6bg .................................
MW 451.2
Frameshift mutagenic standard for Ames test in Salmonella and E. Coli. Science, 176, 47 (1972); Ann. N.Y.
Acad. Sci., 269, 21 (1975); J. Med. Chem., 15, 739 (1972); Proc. Nat’l. Acad. Sci., USA, 72 5135 (1975)
2% Acridine Orange, Ready-to-Use HU5d ......................................
DNA intercalating dye. A grade of acridine orange of exceptionally high purity, suitable for quantitative
work. Ready-to-use and free of inorganic salts. A specific stain for RNA, used as a 2% solution.
Ready-to-use format eliminates the exposure to potentially irritating powdered dyes.
J. Histochem. Cytochem., 22, 495 (1974); 31, 737 (1983); 44, 393 (1996); 49, 921 (2001) J. Lab Med., 15(3), 180 (1984)
Auramine O Stain Kit .......................................................
Acid-fast mycobacteria resist decolorization by acid-alcohol after primary
staining owing to the high lipid (mycolic acid) content in their cell walls. The
identification of mycobacteria with auramine O is due to the affinity of the
mycolic acid in the cell walls for the fluorochromes. The dye will bind to the
mycobacteria, which appear as bright yellow luminous rods against a dark
background. The potassium permanganate helps prevent non-specific fluorescence.
All acid-fast organisms will be stained by Auramine O, including some
parasites. Slides stained with auramine O may be restained with Ziehl-Neelsen
or Kinyoun stain directly, as long as the oil has been removed. This provides a
convenient method of confirming and differentiating morphology of positive
slides with the traditional stains. The fluorochromes stains are recommended
for specimen examination because of their increased sensitivity and speed.
Technical Data Sheet #751
M. tuberculosis in a sputum
smear is stained using fluorescent
auramine with acridine
orange counterstain; 950x.
CDC Public Health Library.
Bisbenzimide (Hoechst 33258) [23491-45-4] H4abd . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
MW 623.97
C H CI N O 27 37 3 6 4
Fluorescent chromosome stain. Recommended use is 10mg/ml for 2-10
minutes. This will vary based on section thickness.
Science, 220, 620 (1983), Anal. Biochem., 179, 401 (1989)
Rat Brain Sagittal, 8 micrometer
section, stained with Hoechst
33342, Alexa Fluor 568-6FAP
(Rb) and Alexa Fluor 488-NF-P
(Ms). Photo: Mike Davidson of
Florida State University
Calcein, 2,4-Bis(N,N’-di[carboxymethyl]aminomethyl) fluorescein [1461-15-0] U5f ...
MW 622.65
Fluorescent bone stain. Also used for determination of serum magnesium. Stain Technology, 62, 247, (1987)
Coumarin 1 [91-44-1] A2g ...................................................
(7-Diethylamino-4-methylcoumarin; 7-Diethylamino-4-methyl-1-benzo-pyran-2-one.)
MW 231.3 Exc. max: 373nm Emiss. max: 460nm
Fluorescent dye, miscible in ethanol or neutral buffer.
Coumarin 6, laser grade U7g .................................................
MW 350.44 Absorbtion max (in ethanol): 458nm Tunable from 515-585nm.
Optimum lasing (in ethanol): 540nm Fluorescence max (in ethanol): 505nm. For pulsed and CW
operation in the green-blue spectral region.
Catalog # Size
04539-500 500 mg
04539-5 5 g
08704-100 100 mg
24603-10 10 ml
24665-1 1 kit
09460-100 100 mg
03321-1 1 g
24185-250 250 g
8037L-100 100 mg
For more information please call (800) 523-2575 or visit: polysciences.com 81