BioSciences - Polysciences, Inc.

Electron Microscopy
BioSciences
Thiocarbohydrazide [2231-57-4] HPW7g .......................................
MW 102.15 mp 171° (dec.) Requires Poison Pack
Useful for the histochemical and ultracytochemical demonstration of glycomacro-molecules. Also
useful for the enhancement of the fine structure of cytomembranes by osmium-thiocarbohydrazide
bridging. Carbonothioic dihydrazide; Thiocarbazide Technical Data Sheet #184
J. Histochem. Cytochem., 29, 682 (1981); 32, 455 (1984)
Uranyl Acetate 98%, ACS Reagent [6159-44-0] HP6g ............................
MW 424.15
Uranyl acetate improves tissue penetration and contrast without affecting
immunolabeling. Useful as positive or negative stain for thin sections.
EM J. Cell Biology, 101, 84a (1985)
Rat skeletal muscle. Stained with Uranyl
acetate and lead citrate. Courtesy, Dr. J. Fulthorpe,
Dept. Muscular Dystrophy Research,
Newcastle General Hospital, UK.
Uranyl Formate [16984-59-1] PS7g ............................................
d 3.7g/cc Appearance: solid yellow crystals UO 2 (CHO 2 ) 2 - H 2 O
Electron dense, heavy metal compounds of uranium, tungsten and molybdenum
have long been utilized for “negative staining” in electron microscopy. As demand
for ultra-fine structure in biomolecules has increased, new fine grain structure
materials are required. The fine grain structure, fast biomedical tissue fixation properties
and stability of this material make it unique for many imaging protocols.
Negative stain electron microscopy of the integrin a5b1 headpiece
with and without a bound fibronectin (Fn) fragment containing Fn
domains 7 to 10 (Fn9-10). a: Negative stain electron microscopy
reveals that the ·5‚1 headpiece adopts two conformations, namely
a closed (black circles) and an open conformation (white circles).
Micrographs courtesy of Tom Walz, Harvard Medical School.
Catalog # Size
01211-5 5 g
01211-25 25 g
21447-25 25 g
24762-1 1 g
24762-5 5 g
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