BioSciences - Polysciences, Inc.
BioSciences - Polysciences, Inc.
BioSciences - Polysciences, Inc.
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
74<br />
Electron Microscopy<br />
<strong>BioSciences</strong><br />
Neutral red, C.I. 50040, certified [553-24-2] U5g ................................<br />
(Basic red 5; 3-Amino-7-dimethylamino-2-methylphenazine hydrochloride; Toluidine red)<br />
MW 288.78 l max 540nm<br />
Used as an indicator. pH range 6.8 (red)-8.0 (yellow). Also used as a biological stain.Merck Index 11, 6395<br />
Nitroblue tetrazolium chloride (NBT) [298-83-9] HU6ae ..........................<br />
MW 817.65 mp 215-217°<br />
Used for estimating dehydrogenases and other oxidases. Also used in the detection<br />
of nucleic acid hybridization and in the detection of ascorbate peroxidase<br />
activity in native gels. Totally soluble and formazan free.<br />
Methods Enzymol., 6, 958 (1963); Anal. Biochem., 56, 353 (1973); 212, 540 (1993), J. Clin. Lab.<br />
Anal., 7, 174 (1993)<br />
Nonenyl Succinic Anhydride (NSA), EM Grade [28928-97-4] H4g ..................<br />
MW 224<br />
Nonenyl Succinic Anhydride: Liquid anhydride for curing low viscosity embedding<br />
resin for EM, particularly used in Spurr formulations. Our material is specially<br />
distilled for E.M. applications and was developed in conjunction with Dr. Spurr.<br />
J. Biochem., 113, 573 (1993)<br />
Phosphomolybdic acid hydrate, ACS grade [12026-57-2] BG6d ....................<br />
MW 3939.45<br />
Electron dense metal stain.<br />
Phosphotungstic Acid hydrate [12501-23-4] B4g ................................<br />
MW 2880.2<br />
Negative Stain, EN Bloc Stain. Principles and Techniques of E. M., 3rd ed., CRC Press, p. 273-279<br />
Pyroxylin, 23% solids solution in ethyl acetate CHV7g ..........................<br />
Used for making thin films for support grids.<br />
Ruthenium hexammine trichloride [14282-91-8] H4g ............................<br />
MW 309.61<br />
Electron microscopy stain. J. Histochem. Cytochem., 31, 717 (1983)<br />
Spurr Low Viscosity Embedding Kit HMV7g ....................................<br />
Exceptional penetration for tissue, mineral, and dense structures. A low viscosity<br />
of 60cps allows easy penetration into a variety of difficult materials. May<br />
be used to prepare mineral specimens for polishing. The low viscosity of the<br />
Spurr formulation allows rapid infiltration into tissues, minerals and other dense<br />
structures. Facilitates embedments with high lipid contents or hard membranes<br />
or highly vacuolated parenchymatous tissues. Technical Data Sheet #127<br />
Kit Contains: 450g NSA, 250g ERL 4221, 250g D.E.R. 736, 100g DMAE<br />
For more information please call (800) 523-2575 or visit: polysciences.com<br />
Microscopic blood clot in<br />
glomerulus of the human kidney<br />
after transplant. Courtesy of<br />
P.Wills, Harris Medical Labs<br />
Spurr Low Viscosity Mini Kit HMV7g ..........................................<br />
Exceptional penetration for tissue, mineral, and dense structures. A low viscosity of 60cps allows<br />
easy penetration into a variety of difficult materials. May be used to prepare mineral specimens for<br />
polishing. The low viscosity of the Spurr formulation allows rapid infiltration into tissues, minerals,<br />
and other dense structures. Facilitates embedments with high lipid contents or hard membranes or<br />
highly vacuolated parenchymatous tissues. Technical Data Sheet #472D<br />
Kit Contains: 55ml NSA, 20ml ERL-4221, 12ml DER736, 1ml DMAE, 3 x 10ml syringes, 1 x 5ml<br />
graduated syringe and 2 wooden mixers<br />
Catalog # Size<br />
00915-25 25 g<br />
00928-500 500 mg<br />
00928-1 1 g<br />
01542-450 450 g<br />
01542-4 4 x 450 g<br />
01021-25 25 g<br />
23224-25 25 g<br />
06456-50 50 g<br />
17253-1 1 g<br />
01916-1 1 kit<br />
21961-1 1 kit
Change language