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BioSciences - Polysciences, Inc.

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Electron Microscopy<br />

EM Reagents<br />

<strong>BioSciences</strong><br />

Chloroauric acid [16961-25-4] H5bd ...........................................<br />

MW 393.8 d 3.90<br />

Used for production of colloidal gold solutions.<br />

Cobalt (II) chloride, EM grade [7646-79-9] HX7g .................................<br />

Used to intensify DAB reaction product. J. Histo. Cyto., 30, 1097 (1982); 29, 775 (1981)<br />

Colchicine [64-86-8] BHX6g ...................................................<br />

MW 399.4 mp 155-157° Requires Poison Pack<br />

An antimicrotubular effector. Interferes with microtubule organization by binding specifically with<br />

tubulin. Used for research in plant genetics.<br />

Microtubules 11 (1978), Springer-Verlag, Berlin<br />

ERL-4221 [2386-87-0] HO4g .....................................................<br />

(3,4 Epoxy Cyclohexyl Methyl 3,4 epoxy Cyclohexyl Carboxylate)<br />

MW 252.3 g/mole bp (760 mm Hg) > 250°C in Water: (weight % at 20k°C)<br />

< 0.06% Appearance: transparent, low color viscous liquid C 14 H 20 0 4<br />

Freezing Point: -35ºC Specific Gravity: (20ºC)~ 1.17<br />

ERL-4221 is a cycloaliphatic, diepoxy functional organic compound that is a<br />

useful building block in the production of semi-hard to hard cured epoxy resins.<br />

It is incompatible with amines, acids and strong bases and will cure to form a<br />

resinous product. ERL-4221 is often used in manufacture of plastic embedding<br />

kits for electron microscopy.<br />

Formvar 15/95 (Poly[vinyl formal]) [9003-33-2] H4g<br />

MW 24,000-40,000 n20<br />

D 1.500 Requires Poison Pack Tg 108° powder TSCA<br />

Powder used to make support films on E.M. grids for fragile samples. Polymer contains cyclic acetal<br />

units, some residual hydroxyl groups. Soluble in: acetic acid, dioxane, THF, chloroform.<br />

Catalog # Size<br />

Formvar 15/95 (Poly[vinyl formal]) 00631-100 100 g<br />

Formvar 15/95 (Poly[vinyl formal]), 0.5 wt%<br />

Solution in 1,2-Dichloroethane<br />

04672-100 100 g<br />

Lead Citrate Trihydrate [512-26-5] HM5g .......................................<br />

MW 1053.8<br />

The most popular lead stain for EM applications.<br />

J. Cell Biol., 17, 208 (1963); J. Cell Biol., 25, 407 (1965)<br />

Lead Citrate, Powdered Fines Grade (< 5 µm) [512-26-5] HM5g ...................<br />

MW 1053.8 Powdered<br />

The most popular lead stain for EM applications. Carbonate free material.<br />

Reynolds, J. Cell Biol., 17, 208 (1963) Venable and Coggeshall, J. Cell Biol., 25, 707 (1965)<br />

Lead (II) Nitrate, 99% (ACS Reagent) [10099-74-8] BHV7g ........................<br />

MW 331.20 d 4.53<br />

Used by Reynolds to make Lead Citrate, carbonate free.<br />

Stains and Cytochemical Methods, Plenum Press, 223 (1993)<br />

Multiple Stain Solution (Paragon Stain) C5g ...................................<br />

Used in Tzanck preparations of herpetic lesions and differentiates acidophilic<br />

and basophilic structures. Multiple Stain is a replacement for the former Paragon<br />

Multiple Stain (PMS). Can be directly applied to frozen sections, epoxy,<br />

JB-4 ® embedded sections or paraffin. It is utilized as a general cytoplasmic<br />

and nuclear stain. Multiple Stain also differentiates various cytologic processes<br />

including basal cell carcinoma, squamous cell carcinoma, malignant melanoma,<br />

B-cell lymphoma, acute myelomonocytic leukemia and metastatic breast cancer.<br />

Multiple Stain Solution is an easy to use, one step procedure with H & E<br />

quality staining. Technical Data Sheet #269<br />

Kidney stained with Multiple<br />

Stain Solution in Peel-A-Way ®<br />

Micro-Cut Paraffin, 4u, 10X<br />

00395-1 1 g<br />

04930-100 100 g<br />

00345-500 500 mg<br />

24738-250 250 g<br />

00378-100 100 g<br />

25350-100 100 g<br />

00785-100 100 g<br />

08824-50 50 ml<br />

08824-100 100 ml<br />

For more information please call (800) 523-2575 or visit: polysciences.com 73

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