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BioSciences - Polysciences, Inc.

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Electron Microscopy<br />

<strong>BioSciences</strong><br />

Catalog # Size<br />

Square Mesh Grids - ThickThin Pattern - Copper 200mesh 7779C-1 100 grids<br />

Square Mesh Grids - ThickThin Pattern - Copper 600mesh 7780C-1 100 grids<br />

Square Mesh Grids - ThickThin Pattern - Nickel 600mesh 7780N-1 100 grids<br />

Grids - Storage Units<br />

Specimen grid box for holding 100 grids in numbered positions. Transparent sliding cover has slot<br />

for accessing the grid positions.<br />

Grid Storage Units<br />

Grid Storage Units - 100 grids 100 grids 03606-1 1 unit<br />

Grid Storage Units - 100 grids 100 grids, 10 pack 03606-10 10 units<br />

Grid Storage Unit - 50 grids 50 grids, 5 pack 23146-5 5 units<br />

50 grids, 25 pack 23147-25 25 units<br />

Grids - Accessories<br />

Grid Coating Plate ..........................................................<br />

Useful in preparing Carbon and Formvar support films on grids. Up to 50 grids can be located<br />

on the Nickel plate and the liquid level lowered onto the grids.<br />

Immunogold Labeling Reagents<br />

Colloidal Gold Solution, 0.005% U2w<br />

Colloidal gold has been widely used to make bioconjugate probes for labeling and visualizing biologic<br />

specimens via light and electron microscopy. Most proteins can be easily coupled to colloidal<br />

gold particles with retention of the bound protein’s biological activity.<br />

size range<br />

15-25nm 09285-50 50 ml<br />

ImmunoGold labeling reagents for (SEM) A2dmw<br />

PolyGold reagents are of the highest quality and can be relied upon to give reproducible results. For<br />

TEM studies, the most convenient sizes of gold particles are 5nm, 10nm and 15nm. The long shelf<br />

life (12 months when stored at 4°C) makes the use of these reagents economical. Resolution of<br />

most SEMs is such that immunolabeling studies require either the use of larger sized gold particles<br />

or enhancement of smaller gold particles using silver enhancement (deposition) technology. Accordingly,<br />

the gold colloids listed below include 20nm, 30nm and 40nm particles for direct visualization<br />

in the SEM.<br />

Benefits:<br />

• High specificity<br />

• Low clustering – agglomeration of gold particles is minimal, over 85% of particles being singlets<br />

• Discrete particle sizing – narrow particle size distribution allows double labeling to be achieved<br />

08511-1 1 plate<br />

Unconjugated gold colloid (GC) 20nm 22719-100 100 ml<br />

Unconjugated gold colloid (GC), 40nm 22720-100 100 ml<br />

Unconjugated gold colloid (GC), 60nm 22703-100 100 ml<br />

Unconjugated gold colloid (GC), 10nm 22717-100 100 ml<br />

Unconjugated gold colloid (GC), 15nm 22718-100 100 ml<br />

Unconjugated gold colloid (GC), 5nm 22716-100 100 ml<br />

For more information please call (800) 523-2575 or visit: polysciences.com 71

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