BioSciences - Polysciences, Inc.

Histology & Microscopy
BioSciences
Picrosirius Red Stain Kit .....................................................
Collagen Type I and III Staining
Our Picrosirius Red Stain binds specifically to collagen fibrils of varying diameter
that is used to distinguish collagen Type I from Type III. Picrosirius Red
Stain will quantify the amount of collagen in a given area of myocardial
tissue. (i.e. the collagen area fraction). Collagenous structures of the mandible
stain brilliant red. Unlike sections stained with hematoxylin and eosin
alone, dentinal tubules, Sharpey’s fibers and other structures can be seen
clearly after using Picrosirius Red Stain procedure. Under polarized light, collagen
fibers can be specifically identified and their orientation determined.
Puchtler et al., 1973; Junqueira et al., 1979
Poly G Blue, Ready-to-Use Stain CH4ag ........................................
Poly R Blue, Ready-to-Use Stain CH4ag
Poly G and R Blue are ready-to-use Coomassie ® G250/R250 blue stain solutions
for protein electrophoresis staining applications. Convenient 500ml size saves
time and preparation costs. Ready-to-use format eliminates the exposure to
potentially irritating powdered dyes.
Prussian Blue Iron Stain Kit (Reaction for Demonstration of Iron) H4g .............
Prussian Blue or Perls’ reaction is used to demonstrate ferric iron and ferritin.
This is not a true staining technique rather, it is a histochemical reaction. The
protein is split off by the hydrochloric acid, allowing the potassium ferrocyanide
to combine with the ferric iron. This forms the ferric ferrocyanide or Prussian
Blue. Technical Data Sheet #601
Rapid Mucin Stain Kit .......................................................
Secretions of mucins are produced in several areas including epithelial and
connective tissue. Inflammation can cause secretion of mucin as well as some
types of intestinal carcinomas. The use of a Rapid Mucin stain will quickly determine
the presence of mucin and assist in the direction of other special stains
or immunohistochemistry to determine the origin of the mucin producing cells.
The entire kit procedure takes 12 to 15 minutes after paraffin removal.
Technical Data Sheet #600
Human kidney tissue stained
with Prussian Blue iron Stain
Human colon tissue. stained
with Rapid Mucin Stain Kit.
Ruthenium red [11103-72-3] H5g .............................................
MW 858.42
Stain for polysaccharides and for elastic fiber in tissue. Also a myoneural junction stain.
J. Cell Biol., 57, 874 (1973)
Saffron [42553-65-1] ........................................................
Saffron is used in a variety of histological staining methods including the Hematoxylin, Phloxine
and Saffron (HPS) staining technique. The HPS stain demonstrates collagen in connective tissues.
Hematoxylin, stains acidic structures such as DNA, purple. Phloxine, stains most proteins pink and
Saffron stains collagen yellow.
Silver Enhancement Kit for LM B4d ...........................................
Two component kit for use with both 1nm & 5nm immunogold labels which gives excellent results
with silver enhancement. Adequate amplification is usually obtainable in a period of 5-10 minutes
and can be monitored on a microscope. The enhancement is performed by mixing together one
drop of each solution on a slide.
Silver Nitrate [7761-88-8] BG6g ...............................................
MW 169.89
Equal to or better than ACS specifications. Silver stain techniques are widely used to detect nanogram
quantities of proteins following electrophoresis. Silver nitrate is the silver source in most silver
stain procedures. Assay: 99.9% min. Anal. Biochem., 156, 96 (1986)
Catalog # Size
24901-250 250 ml
24901-500 500 ml
24609-500 500 ml
24608-500 500 ml
24199-1 1 kit
24208-1 1 kit
01098-1 1 g
25007-25 25 g
22708-1 1 kit
01125-25 25 g
01125-100 100 g
For more information please call (800) 523-2575 or visit: polysciences.com 57