BioSciences - Polysciences, Inc.

54
Histology & Microscopy
Staining - Histology / Cytology
Dyes and Stains for Histology
BioSciences
Alcian Blue/PAS Kit .........................................................
Demonstrates neutral and acidic mucosubstances on tissue at pH 2.5 and
imparts a blue color to the acidic mucins and other carboxylated or weakly
sulphated acid mucosubstances. Periodic Acid Schiff (PAS) reaction is then used
to stain basement membranes, glycogen and neutral mucosubstances pink to
red. Mixtures of neutral and acidic mucosubstances will appear purple due to
positive reactions with both Alcian Blue and PAS. Technical Data Sheet #886
Ammonium Blue H4g .......................................................
Very rapid formulation for bluing hematoxylins in only 3 minutes. Ammonium
Blue is buffered at an alkalinity of (pH = 10.0). Our premixed and ready-to-use
Ammonium Blue provides crisp nuclear detail obtained with Hematoxylin and
Eosin stains. For use in both Cytology and Histology.
Amyloid Stain Kit (Congo Red) ...............................................
Complete kit to aid in the staining of tissue for Amyloidosis. Amyloid Stain Kit used in the detection
of amyloid FFPE as well as frozen tissue sections cut at 10 microns. The amyloid stains red and the
nuclei stains blue. Control tissue is Alzheimer’s or other known amyloidosis.
Biebrich Scarlet - Acid Fuchsin Solution, Ready-to-Use ...........................
(1% Acetic Acid, .9% Biebrich Scarlet, .1% Fuchsin) For use in Masson Trichrome stain procedure.
Bromophenol Blue, ACS [115-39-9] ............................................
MW 669.96 l max 422nm C 19 H 10 Br 4 O 5 S
Bromophenol blue is useful as an acid-base indicator, a color marker and a dye. As an acid-base
indicator its range lies between pH 3.0 and 4.6. Suitable as a color marker in agarose gel electrophoresis
and polyacrylamide gel electrophoresis and commonly used as a dye to stain proteins in slides.
Merck 13, 1429 Beilstein 19, V, 3, 458
Bromothymol Blue, ACS [76-59-5] . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
MW 624.38 mp 200-202 °C C 27 H 28 Br 2 O 5 S UV Absorption: l max 420nm
Bromothymol blue is a chemical indicator for weak acids and bases, useful for observing photosynthetic
activities or respiratory indicators (turns green then yellow as CO 2 is added).
Merck 13, 1430 Beilstein 19, V, 3, 461
Bromothymol Blue, Sodium Salt, ACS [76-59-5] .................................
MW 646.36 l max 420nm C 27 H 27 Br 2 NaO 5 S
Bromothymol blue is a chemical indicator for weak acids and bases, mostly used in measuring
substances that would have relatively low acidic or basic levels (near a neutral pH). Suitable for
use in the laboratory as a biological slide stain. Merck 13, 1430 Beilstein 19, V, 3, 461
Chlorophenol red [4430-20-0] H4g ............................................
(3,’3-Dichlorophenol sulfone phthalein) MW 423.3 l max 572nm
pH indicator; transition interval from pH 4.8 (yellow) to 6.7 (violet).
Cuprolinic blue (Quinolinic phthalocyanine) [41276-95-3] U7ad ...................
MW 1084.54
Intensely blue cationic dye used for the visualization of RNA and other polynucleotides. Stain (microwave)
used for enteric neurons. Can also used as a counterstain in immunoperoxidase procedures.
Histochem. J., 15, 801 (1983); 15, 1113 (1983); Biotechniques, 7, 692 (1989)
Fast Frozen Stain Kit ........................................................
Rapid staining method for frozen sections. Process is suitable for Laser Capture
Microdissection (LCM). Technical Data Sheet #716
Kit Contains: Fast Fix Frozen Fixative, Gill’s Hematoxylin #3, Eosin Y 0.5%,
Scott’s Bluing Reagent and CitraMount Mounting Media.
For more information please call (800) 523-2575 or visit: polysciences.com
Human Pancreas, 20X
Harris Hematoxylin, Eosin Y
Catalog # Size
25086-1 1 kit
24819-1 1 gal
24614-1 1 kit
24602-100 100 ml
24995-5 5 g
24995-25 25 g
25009-25 25 g
25009-50 50 g
24996-25 25 g
03266-10 10 g
17052-100 100 mg
17052-500 500 mg
24604-1 1 kit