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BioSciences - Polysciences, Inc.

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Histology & Microscopy<br />

<strong>BioSciences</strong><br />

Lowicryl ® HM 20 Non-polar, hydrophobic, -70ºC Embedding Kit HIR7ag ............<br />

Lowicryl ® HM kits are non-polar and are used to produce high contrast images<br />

of completely unstained thin sections by Z-contrast. Also these HM kits are<br />

particularly suitable for dark field observation because of their relatively low<br />

density. Technical Data Sheet #248<br />

Kit Contains: 3 x 225gm of monomer E, 1 x 130gm of crosslinker D and<br />

1 x 4.8gm of initiator C<br />

Lowicryl ® K4M Polar Kit, hydrophilic, -35ºC embedding kit HIR7ag ................<br />

Lowicryl K kits are polar and therefore hydrophilic. During dehydration and<br />

infiltration specimens may be kept partially hydrated since polymerization can<br />

occur with up to 5% water. Thus immunolabeling with polar kits results in better<br />

preservation of structure and antigenicity and lower background labeling.<br />

Technical Data Sheet #248<br />

Kit Contains: 1 x 130gm of crosslinker A, 3 x 250gm of monomer B, and<br />

2 x 4.8gm of initiator C<br />

MonoStep Lowicryl ® HM-20 Non-polar Embedding Media CHO6d ...............<br />

MonoStep Lowicryl ® K4-M Polar Embedding Media BHO6d .....................<br />

Pre-mixed, ready to use Lowicyrl ® MonoStep Embedding Media saves time and minimizes<br />

chemical contact. These products are ideal for use in Immunohistochemistry. They are based on<br />

our popular Lowicyrl ® K4M and HM-20 formulations for low temperature embedding or freeze<br />

substitution. MonoStep products are especially appropriate for immunolableing resulting in<br />

better preservation of antigenicity and lower background labeling.<br />

MonoStep Lowicryl ® K4-M Polar Embedding Media BHO6d .....................<br />

Pre-mixed, ready to use MonoStep Lowicyrl ® Embedding Media saves time and minimizes chemical<br />

contact. These products are ideal for use in Immunohistochemistry. They are based on our popular<br />

Lowicyrl ® K4M and HM-20 formulations for low temperature embedding or freeze substitution.<br />

MonoStep products are especially appropriate for immunolableing resulting in better preservation<br />

of antigenicity and lower background labeling.<br />

Osteo-Bed Bone Embedding Kits<br />

Osteo-Bed Bone Embedding Kit GH5d ........................................<br />

Formulated for embedding large and small mineralized (undecalcified) bone specimens. Large bone<br />

samples embedded with Osteo-Bed should be sectioned with a heavy-duty microtome. Small bone and<br />

soft tissue should be sectioned with a microtome designed to cut plastic embedded materials. Osteo-<br />

Bed is a methyl methacrylate-based material that can be removed from the section and allows the use<br />

of staining procedures very much like a paraffin section. Also recommended for immunohistochemistry<br />

procedures performed on trephines and other non-decalcified bone or soft tissues. Not water-soluble.<br />

Osteo-Bed Bone Embedding Resin Solution A CHO5g ............................<br />

Osteo-Bed Bone Embedding Benzoyl Peroxide Plasticized (Catalyst) GH5g .........<br />

Osteo-Bed Bone Embedding Solvent HVWX6g ..................................<br />

Osteo-Bed Plus Embedding Kit CGHO5g .......................................<br />

Osteo-Bed Plus Embedding Kit offers the same benefits as Osteo-Bed Bone<br />

Embedding Kit with the added benefit of producing much harder blocks for<br />

supporting undecalcified bone specimens and bone containing metal implants,<br />

grafts and stents. Suitable for use with large and small mineralized (undecalcified)<br />

bone sections and hard tissues.<br />

Benefits:<br />

• Yields clear, hard blocks for cutting sections with a heavy duty microtome<br />

• Infiltrates rapidly and produces high hardness after polymerization<br />

• Try Osteo-Bed Bone Embedding Solvent (Cat. #17734B) to remove<br />

plastic from sections for brilliant staining<br />

Catalog # Size<br />

15924-1 1 kit<br />

15923-1 1 kit<br />

23994-225 225 g<br />

23646-225 225 g<br />

23646-225 225 g<br />

17734-1 1 kit<br />

17734A-900 900 ml<br />

17734C-2 2X12 g<br />

17734B-4 4x500 ml<br />

24889-1 1 kit<br />

For more information please call (800) 523-2575 or visit: polysciences.com 49

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