BioSciences - Polysciences, Inc.

48
Histology & Microscopy
BioSciences
Spurr Low Viscosity Embedding Kit HMV7g ....................................
Exceptional penetration for tissue, mineral, and dense structures. A low viscosity
of 60cps allows easy penetration into a variety of difficult materials. May
be used to prepare mineral specimens for polishing. The low viscosity of the
Spurr formulation allows rapid infiltration into tissues, minerals and other dense
structures. Facilitates embedments with high lipid contents or hard membranes
or highly vacuolated parenchymatous tissues. Technical Data Sheet #127
Kit Contains: 450g NSA, 250g ERL 4221, 250g D.E.R. 736, 100g DMAE
Spurr Low Viscosity Mini Kit HMV7g ..........................................
Exceptional penetration for tissue, mineral, and dense structures. A low viscosity of 60cps allows
easy penetration into a variety of difficult materials. May be used to prepare mineral specimens for
polishing. The low viscosity of the Spurr formulation allows rapid infiltration into tissues, minerals,
and other dense structures. Facilitates embedments with high lipid contents or hard membranes or
highly vacuolated parenchymatous tissues. Technical Data Sheet #472D
Kit Contains: 55ml NSA, 20ml ERL-4221, 12ml DER736, 1ml DMAE, 3 x 10ml syringes, 1 x 5ml
graduated syringe and 2 wooden mixers
Ultra Low Viscosity Embedding Kit EHOV7g ....................................
ERL-4221 is useful for rapid embedding of materials which are difficult to infiltrate even with
Spurr’s resin. Exhibits good sectioning qualities, beam stability, and staining properties. Miscible
with alcohol or acetone so propylene oxide can be avoided. For EM and LM - NOT water soluble.
Technical Data Sheet #354
Kit Contains: 1 x 100g ERL-4221, 2 x 100g Octenyl Cuccinic Anhydride (DSA), 1 x 25g 1,4-Butanediol
Diglycidyl Ether (BDE) and 1 x 100g Dimethylaminoethanol (DMAE).
Lowicryl ® Embedding Kits
Manufactured by Polysciences, these kits were developed in cooperation with the University of
Basel, Switzerland. MonoStep K4M and MonoStep HM20 offer a convenient single bottle of material
for Polar -35°C and Non-polar -70°C applications. Photopolymerization is by long wavelength
(360nm) UV. Polymerization can take place at -70°C or at room temperature with UV light. Lowicryl
gives enhanced preservation of protein molecules wand membrane structures. It is a methacrylate
based, UV curing resin. All kits are useful for freeze-substituted samples. Resins should be used at
maximum temperature of -20°C to minimize disruption of proteins. Lowicryl embedments have
been reported to have rougher surfaces than epoxy embedments yielding more antigen at the section
surface. Metal stains with Lowicryl are superior to uranyl and lead combinations.
Technical Data Sheet #248 J. EM Techniques, 11, 1099 (1989); 18, 172 (1991); J. Histochem. 40, (1), 123 (1992)
Lowicryl ® HM 23 Non-polar, hydrophobic, -80ºC embedding kit CHIR7c ............
Lowicryl ® HM kits are non-polar and are used to produce high contrast images of completely unstained
thin sections by Z-contrast. Also these HM kits are particularly suitable for dark field observation
because of their relatively low density. Technical Data Sheet #248
Kit Contains: 3 x 225 gm of monomer G, 1 x 40gm of crosslinker F, 1 x 3.7gm of initiator C and
1 x 5.5gm of initiator J. Instructions included.
Lowicryl ® K11M Polar Kit, hydrophilic, -60ºC embedding kit ......................
Lowicryl ® K kits are polar and therefore hydrophilic. During dehydration and infiltration
specimens may be kept partially hydrated since polymerization can occur with up to 5%
water. Thus immunolabeling with polar kits results in better preservation of structure and
antigenicity with lower background labeling. Technical Data Sheet #248
Kit Contains: 3 x 250gm of monomer I, 1 x 40gm of crosslinker H and 1 x 4.3 gm of initiator C.
For more information please call (800) 523-2575 or visit: polysciences.com
Microscopic blood clot in
glomerulus of the human kidney
after transplant. Courtesy
of P.Wills, Harris Medical Labs
Catalog # Size
01916-1 1 kit
21961-1 1 kit
17706-1 1 kit
18162-1 1 kit
18163-1 1 kit