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BioSciences - Polysciences, Inc.

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Histology & Microscopy<br />

Plastic Embedding Media & Kits<br />

<strong>BioSciences</strong><br />

Araldite resins (modified epoxy resins)<br />

Araldite 502 Kit, Luft’s Formula EHJO7g .......................................<br />

Araldite 502 kit, Luft’s Formula overcomes penetration difficulties by using propylene<br />

oxide as an additional clearing agent. Permits embedding of tissue from<br />

fixation to sectioning in about 24 hours. Technical Data Sheet #128<br />

Kit Contains: 500g Araldite 502, 450g DDSA, 100g DMP-30, instruction sheet<br />

J. Biophys. Biochem. Cytol., 9, 409 (1961)<br />

Araldite 502/PolyBed 812 Kit EHJO7g .........................................<br />

Mollenhauer suggested that hardness variation and penetration enhancement could be obtained<br />

by mixing Araldite and Epon resins. We offer Araldite 502 and PolyBed 812 for researchers to utilize<br />

Mollenhauer’s findings. PolyBed 812 is an exact chemical match for Epon 812. Both Araldite 502<br />

and Polybed 812 are useful as an embedding medium with acetone or alcohol dehydration.<br />

Kit Contains: 500g PolyBed 812, 500g Araldite 502, 450g DDSA, 100g DMP-30, instruction sheet<br />

Technical Data Sheet #128 Stain Tech., 39, 111 (1963)<br />

Catalog # Size<br />

Araldite resins (modified epoxy resins) [84-74-2] HO5g<br />

TSCA C 6 H 4 -1,2,-[CO 2 (CH 2 ) 3 CH 3 ] 2<br />

WPE is Weight Per Epoxide equivalent. From this value, one determines the optimum amount of<br />

DDSA or NMA in grams to combine with 100 grams of resin for stoichiometric balance minimizing<br />

unreacted starting materials and producing reproducibly stainable, embedding blocks. The exact<br />

WPE value for each lot of Araldite resin is put on the label of each container.<br />

Technical Data Sheet #128<br />

Grade WPE<br />

Araldite resins (modified epoxy resins), Grade 502 502 233-250 00552-500 500 g<br />

Araldite resins (modified epoxy resins), Grade 6005 6005 182-189 02116-500 500 g<br />

Mixing Chart for Araldite ® Resins, DDSA and NMA<br />

Per 100g of epoxy<br />

as supplied at WPE<br />

Mix this many<br />

grams of (soft mix) DDSA<br />

Mix this many grams of<br />

(hard mix) NMAA<br />

170 85 78<br />

175 83 76<br />

180 81 74<br />

185 78 72<br />

190 76 70<br />

195 74 68<br />

200 73 66<br />

205 71 65<br />

210 69 63<br />

215 67 62<br />

220 66 60<br />

225 64 59<br />

230 63 58<br />

235 62 57<br />

240 60 55<br />

245 59 54<br />

250 58 53<br />

The hard mix and the soft mix are then blended according to the desired<br />

hardness of the particular embedment procedure.<br />

02600-1 1 kit<br />

02595-1 1 kit<br />

For more information please call (800) 523-2575 or visit: polysciences.com 45

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