BioSciences - Polysciences, Inc.

Immunohistochemistry (IHC)
BioSciences
Neuron specific enolase, Rat, lyophilized, purity >99% A2d ......................
Neuron specific enolase (NSE) is a unique form of the glycolytic enzyme enolase (E.C.4.2.1.11)
found in neurons and in virtually all of the neuroendocrine, paraneuronal cell types. Certain neurological
disorders are accompanied by increases in serum and spinal fluid NSE levels. NSE is also found
in high concentrations in neuroendocrine tumors. Levels of NSE in biological fluids can be useful
parameter for the assessment of neural tissue damage in the brain. Our human NSE and rat NSE
have a specific activity of 40-60 units per mg. Both can be used in conjunction with anti-human NSE
or anti-rat NSE to formulate an RIA to measure NSE levels in tissue, serum, or spinal fluid.
Technical Data Sheet #314
J. Neurochem., 36, 1093 (1981); Ann. Rev. Neurosci., 10, 269 (1987); Int. J. Dev. Neurosci., 6, 77 (1988).
Rabbit IgG, whole molecule, lyophilized (Purified protein) H4d ...................
Rabbit IgG, whole molecule, lyophilized.
New! IHC Hematoxylin/Bluing Kit ............................................
Formulated to impart light nuclear counterstaining desired with immunohistochemical chromogen
staining. Controlled nuclear counterstaining will not over-stain and obscure nuclear IHC staining.
Ready to use. Volume: 500ml for A and B.
Kit contains: Counterstain and bluing solution
Reagents - IHC
DAB-4HCL (3-3’-Diaminobenzidine tetrahydrochloride) Immunochemical Grade [7411-49-6] HMVW7bg
MW 360.1
Chromogen of choice for immunoperoxidase techniques. The brown reaction
product is highly insoluble, and will not diffuse from the site of localization. The
color does not fade, and therefore, slides can be saved as a permanent record.
The reaction product also chelates with osmium tetroxide and becomes electron
dense for use in electron microscopy. By adding buffer directly to the DAB
serum vial with a hypodermic syringe, contact with the solid DAB is avoided.
Our DAB-4HCl is prepared to the highest purity standards. It is fully water soluble
and may be used without further purification for immunocytochemistry and
for endogenous oxidases and peroxidase. Technical Data Sheet #164
Manual of Immunoperoxidase Techniques, 2nd Edition, ASCP Press, Chicago, 1987
Human breast tissue stained
with DAB in Peel-A-Way ®
Micro-Cut Paraffin, 100X
New! Guanidine Isothiocyanate Solution, 4M [593-84-0] .........................
Used in purification of RNA. Beilstein Registry #3563461 L.A. Cox, G. S. Adrian Biochemistry 32, 4738, (1993)
New! Guanidine Isothiocyanate Solution, Ultrapure [593-84-0] ...................
Purity: 99.0% pH: (1M in water) 5.0-7.0
Used to isolate RNA from sources like pancreas and high molecular DNA from procaryotic organisms
that require strong enzyme detergent lysis and organic treatment. Strong protein denaturant when
used in high concentrations.
L.A.B. Solution (Liberate Antibody Binding Solution) H4g .......................
L.A.B. Solution liberates your antibody binding sites for improved immunohistochemical
(IHC) staining. Incubate your slides in L.A.B. Solution for 5 to 20 minutes
at room temperature, rinse with buffer and stain. It’s that simple, no high
temperature or special equipment required. Incubating at room temperature
makes L.A.B. Solution compatible with Automated Stainers. Set up a pre-treatment
cycle with L.A.B. Solution before your staining protocol, then walk away.
Technical Data Sheet #630
Benefits:
• Ready to use solution, no dilution necessary
• Turn off the heat and preserve tissue - incubate samples at room temperature
• No special equipment required
• Compatible with Automated Stainers
Catalog # Size
17435A-50 50 µg
23874-10 10 mg
25082-1 1 kit
04001-2 2x25 mg
60 ml vials
04008-5 5x10 mg
5x30 ml
serum vials
04001-5 5x25 mg
60 ml vials
25070-500 500 ml
25071-500 500 g
24310-500 500 ml
For more information please call (800) 523-2575 or visit: polysciences.com 35