BioSciences - Polysciences, Inc.
BioSciences - Polysciences, Inc.
BioSciences - Polysciences, Inc.
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Histology & Microscopy<br />
<strong>BioSciences</strong><br />
Anti-Neuron Specific Enolase (NSE) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .<br />
Both antisera are very specific and are the reagents of choice for the visualization<br />
of neurons and peptide secreting neuroendocrinecells by immunocytochemistry.<br />
The anti-human NSE can be used for immunocytochemical<br />
staining at dilutions of 1:3,000 to 1:5,000. The anti-rat NSE can be used<br />
at dilutions of 1:2,000 to 1:4,000. These antisera are also useful for the<br />
quantification of neurons and peptide secreting neuroendocrine cells by<br />
Radio Immuno Assay (RIA). The anti-human NSE and anti-rat NSE can be<br />
used in conjunction with pure human NSE (Cat. #17436) and pure rat NSE<br />
(Cat. #17435A) to formulate an RIA to measure NSE levels in tissue, serum<br />
or spinal fluid. Technical Data Sheets #266 and #314<br />
Brain Res., 181, 391 (1980); 190, 195 (1981); J. Neurochem., 36, 1093 (1981)<br />
Anti-Human Neuron specific enolase (NSE) in rabbit, serum, lyophilized A3d .......<br />
Anti-rat Neuron specific enolase (NSE) in rabbit, serum, lyophilized A3d ..........<br />
Anti-Lactate dehydrogenase (rabbit muscle) in goat, IgG fraction H4d ............<br />
This Antibody reacts with lactate dehydrogenase from rabbit muscle. All tetrameric isozymes of LDH<br />
react with this antibody. Cross-reactivity will occur against all mammalian forms of this enzyme.<br />
Anti-Ovalbumin (egg white) in rabbit, IgG fraction H4d .........................<br />
Antibody reacts with ovalbumin from hen egg white showing a 45 kDa band under non-reducing<br />
and reducing conditions of SDS-PAGE.<br />
Anti-Pyruvate kinase (rabbit muscle) in goat, IgG fraction H4d ...................<br />
This Antibody reacts with pyruvate kinase from rabbit muscle showing a 237 kDa band (tetramer)<br />
under non-reducing conditions of SDS-PAGE and a 57 kDa band under reducing conditions. Crossreactivity<br />
will occur with most mammalian forms of this enzyme.<br />
Anti-Transferrin (human) in rabbit, IgG fraction H4d ............................<br />
reacts with transferrin from human serum. Strong cross-reactivity will occur with mammalian forms<br />
of this protein. Suitable for: ELISA, Western Blotting, Immunoprecipitation.<br />
Rabbit Anti-Green Fluorescent Protein Antibody H4d ...........................<br />
Green Fluorescent Protein (GFP) was originally discovered in Aequorea victoria, a luminescent<br />
jellyfish. GFP is useful for gene expression studies. Anti-GFP antibodies have<br />
been used for immunohistochemical detection, purification via immunoprecipitation,<br />
protein-protein interaction studies, Western Blotting and ELISA-based assays.<br />
<strong>Polysciences</strong>’ polyclonal Anti-GFP antibody can be used for detection and purification<br />
of GFP tagged proteins and reacts with a number of variants of A.victoria GFP.<br />
Specificity: Reacts with variants of Aequorea victoria GFP, such as S65T-GFP, RS-<br />
GFP and EGFP. Technical Data Sheet #609<br />
Staining of rat cerebral cortical<br />
neurons with anti-rat NSE.<br />
Rabbit Anti-GFP for Immunohistochemical<br />
detection and<br />
immunoprecipitation of chimeric<br />
GFP protiens.<br />
Blocking Reagents<br />
Bovine serum albumin (fraction V, protease-free), lyophilized H4d ................<br />
MW ~66k<br />
Blocking reagent. Lyophilized.<br />
Catalog # Size<br />
17437-100 100 µl<br />
17437-200 200 µl<br />
16625-150 150 µl<br />
16625-350 350 µl<br />
23740-5 5 mg<br />
23744-5 5 mg<br />
23750-5 5 mg<br />
23753-5 5 mg<br />
24240-0.1 0.1 mg<br />
24240-0.2 0.2 mg<br />
23854-10 10 g<br />
For more information please call (800) 523-2575 or visit: polysciences.com 33
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