BioSciences - Polysciences, Inc.

28
Histology & Microscopy
BioSciences
Methanol (Methyl alcohol), 99.5% - EM Grade [67-56-1] CH6g ....................
Dehydrating agent of tissue as a preparation for embedment.
Michel’s Buffer A2mw .......................................................
Holding medium for tissue undergoing immunofluorescent studies. Not to be
used for tissues used in Fluorescent In situ Hybridization (FISH).
Osmium tetroxide, 2% solution - 10 x 2ml BH6g ................................
Post fix and stain for E.M. Microfiltered solution in pre-scored sealed ampoules
with each ampoule having its own ampoule cracker. Technical Data Sheet #119A
Requires Poison Pack.
Osmium tetroxide, 4% solution - 5 x 10ml BH6g ................................
Post fix and stain for E.M. The solution is microfiltered and features exact concentration, no cross
contamination, and has excellent stability when kept cold and in the dark. Supplied in pre-scored
sealed ampoules with each ampoule having its own ampoule cracker. Used for post staining of DAB/
peroxidase to darken and retain stain for long term storage. Technical Data Sheet #119A
Requires Poison Pack.
Manual of Immunoperoxidase Techniques, 2nd Ed., ASCP Press: Chicago, 1987; p58 Electron Microscopic Immunocytochemistry,
Principles and Practice, Eds., Polak and Priestly, Oxford University Press, 1992; p91
Osmium tetroxide, crystalline, 99.95% [20816-12-0] GPRS7g
MW 254.2 mp 39-41°
Post fix and stain for E.M. Supplied in pre-scored ampoules sealed in plastic bags. The ampoules
are label free to avoid cleaning prior to preparation of solutions. Each ampoule is equipped with an
ampoule cracker. Technical Data Sheet #119A Requires Poison Pack.
For more information please call (800) 523-2575 or visit: polysciences.com
Catalog # Size
Osmium tetroxide, crystalline, 99.95% - 5x1g 0223A-5 5x1 g
Osmium tetroxide, crystalline, 99.95% - 10x1/2g 0223C-10 10x1/2 g
Osmium tetroxide, crystalline, 99.95% - 10x1/4g 0223D-10 10x1/4 g
Osmium tetroxide, crystalline, 99.95% - 10x1g 0223B-10 10x1 g
Poly/LEM Fixative HM6g ....................................................
For Light and Electron Microscopy. This popular methanol-free fixative is a formaldehyde-based material
formulated to eliminate the need to select a fixative for tissue based on the type of microscopy to be
employed. Technical Data Sheet #303
PolyTransport Buffer A2g ...................................................
Used to transport and preserve specimens from surgery to the point of grossing and
fixation prior to further processing. PolyTranport Buffer prevents proteins from denaturing
or crosslinking and prevents bacterial growth. PolyTransport Buffer also contains
a cryoprotectant to prevent ice crystal formation during rapid freezing techniques and
a membrane stabilizer to prevent membrane lysis. No washing of specimens necessary
prior to placing in fixative or rapid freezing. Technical Data Sheet #631
Ruthenium tetroxide, 0.5% stabilized aqueous solution [20427-56-9] U5d .........
Useful as a staining agent for electron microscopy of polymers, as well as a fixative
for biological samples. Ruthenium tetroxide penetrates tissue very slowly,
reacting strongly with proteins, glycogen and monosaccharides. Ruthenium
tetroxide can be used as an even more aggressive form of staining for the study
of polymers by TEM than osmium tetroxide. Supplied in pre-scored ampoules
each with its own ampoule cracker. Store at 4°C in refrigerator.
Technical Data Sheet #320 Hayat, M.A., Fixation for E.M., Academic Press, NY, 1981, page 194.
08032-6 6x1 pint
24353-500 500 ml
24353-1 1 gal
23310-10 10x2 ml
23311-10 10X5 ml
0972B-5 5x10 ml
0972C-20 20x10 ml
0972A-20 20x2 ml
16864-3.75 3.75 liters
16864-4 4x3.75
liters
24311-250 250 ml
24311-500 500 ml
18253-5 5x10 ml
18253-10 10x10 ml
18253-25 25x10 ml