BioSciences - Polysciences, Inc.

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Histology & Microscopy
BioSciences
Formaldehydes
New! Flow Fix, 1% Paraformaldehyde Fixative Kit A2d .........................
A sodium azide free buffer comprised of a neutral pH-buffered saline (i.e., Dulbecco’s Phosphate-
Buffered Saline) that contains 1% w/v paraformaldehyde. Sodium Azide –Free. Do not add sodium
azide to buffers if you are concerned with recovering cell function.
New! Flow Fix, 2% Paraformaldehyde Fixative Kit ..............................
Flow Fix, 2% Paraformaldehyde Fixation Buffer is a sodium azide free buffer comprised of
a neutral pH-buffered saline (i.e. -Dulbecco’s Phosphate-Buffered Saline) that contains 2%
w/v paraformaldehyde. Sodium Azide –Free. Do not add sodium azide to buffers if you are
concerned with recovering cell function.
Formaldehyde 37%, U.S.P [50-00-0] BHM6g ....................................
(Formalin 100%) Special handling charge & carrier surcharges.
Formaldehyde, 10%, methanol free, Ultra Pure [50-00-0] BHM6g .................
Suitable for both electron and light microscopy. Easily penetrates large blocks of tissue. When used
in combination with glutaraldehyde, it fixes delicate tissues such as brain in vascular perfusion.
Avoids the problem of having to depolymerize paraformaldehyde. Used in Karnovsky’s fixative in
conjunction with your own buffer system. Source of the formaldehyde is paraformaldehyde.
Polysciences quality
Formaldehyde
Formaldehyde showing
methanol impurity
Formalin, 10% neutral buffered (phosphate buffer) BHM6g ......................
Widely used fixative for processing tissues in routine histology laboratories. Used for IHC in the clinical
laboratory for paraffin embedded tissues. Volume of the fixative should be 15-20 times that of the tissue.
Paraformaldehyde EM Grade [30525-89-4] DH6g ................................
Paraformaldehyde depolymerizes in water to formaldehyde solution yielding
consistent quality fixative solutions. To achieve a strong solution, raise the temperature
of the water to 60ºC then add sodium hydroxide solution dropwise.
Karnovsky, J. Cell Biology, 27, 137A (1965).
Zinc Formalin Fixative, pH 6.25 HVWX6g .......................................
Excellent morphological preservation of nuclear & cytoplasmic components.
Can replace neutral buffered formalin for routine tissue and immunohistochemical
procedures. Non-precipitating fixative which can be used with automated
and manual methods. Zinc formalin is best used as a primary fixative which minimizes
denaturation. Specimens initially fixed in buffered formalin may be post
fixed in Zinc Formalin for a week without an effect. Technical Data Sheet #458
J. Histochem. Cytochem., 31, 1435 (1983)
For more information please call (800) 523-2575 or visit: polysciences.com
Catalog # Size
25037-1 1 kit
25085-1 1 kit
00625-1 1 gal
04018-1 1 liter
04018-4 4x1 liter
18814-20 20x10 ml
08379-3.75 3.75 liters
08379-20 20 liters
00380-250 250 g
00380-1 1 kg
21516-3.75 3.75 liters