BioSciences - Polysciences, Inc.

Histology & Microscopy
Bouin’s Fixative (Bouin’s Fluid) BHM6g
n20
D 1.376 d 1.09
Picric acid, formalin and acetic acid fixative. Bouin’s fixative is excellent for use
in preserving soft and delicate tissue structures. The shrinking induced by the
picric acid is offset by the swelling of the glacial acetic acid.
BioSciences
2,4,6-sym-Collidine Buffer Kit, EM Grade HX7g .................................
A biologically stable buffer system for osmium tetroxide and other EM fixatives.
Provides better stability and buffering capacity than traditional veronal acetate
buffers. One kit will make 1000ml of buffer. Technical Data Sheet #111
Kit Contains: 5 x 5.34ml of Collidine and 5 x 18ml of 1.0N HCl
Bennet, H.S. & Luft, J.H., J. Biophysics & Biochem. Cytol. 6, 113 (1959); Hendrickson, A., et al., Stain
Tech., 43, 175 (1968)
New! Eco-Fix Derm Fixative X7g .............................................
(Formalin Free Tissue Fixative without Alcohol for Dermatology Specimens)
The fixation characteristics of Eco-Fix Derm Fixative are much better than those of formaldehydebased
fixatives, yielding consistent results even after long-term fixation. Carbohydrates, proteins,
antigenic sites and nucleic acids are all effectively preserved. Tissues fixed with Eco-Fix Derm Fixative
have a similar appearance to those fixed in formalin-based fixatives and also show more sensitive
staining results.
Features:
• Specifically formulated for skin samples
• Ideal for Histochemistry, Immunohistochemistry & In Situ Hybridization
• Offers all the features of Eco-Fix Tissue Fixative
New! Eco-Fix MB (Molecular Biology) Tissue Fixative, 1x H3g ....................
Specially formulated to preserve antigenic sites (for antibody probes) and nucleic acid sites (for insitu
hybridizations) in their native state making predigestion or other recovery procedures for these
important sites unnecessary. Replaces common fixatives including: formaldehyde-based, alcoholbased,
Zenker’s, B5 & B3, Bouin’s and other fixatives and provides superior results. Tissues fixed
in Eco-Fix MB exhibit vibrant staining, better nuclear and cytoplasmic detail and will retain a crisp
appearance even after long-term fixation. Eco-Fix MB tissue fixative also retains a much more natural
look and feel than formalin fixed tissue. Softer tissues mean easier, smoother cutting and sectioning.
Features:
• Non-formalin based tissue fixative designed for molecular biology applications
• Preserves antigenic sites and nucleic acid sites in their native state
• Tissue retain natural look and feel compared to formalin fixed tissues
New! Eco-Fix Tissue Fixative .................................................
Eco-Fix Tissue Fixative directly replaces common fixatives including: formalin-based, alcohol-based,
Zenker’s B5, Bouin’s and other fixatives. Can also be used in sequence with other fixatives. The versatility
of Eco-Fix Tissue Fixative makes it optimal for use in histopathology, cytology, histochemistry,
immunohistochemistry and molecular pathology. Preserves tissue integrity, eliminating the need for
marker recovery procedures. Eco-Fix Tissue Fixative is economical to use as it does not require additional
reagents for recovery procedures as do formalin-based fixatives. Also eliminates the need for
expensive ventilation systems and reduces disposal costs. Free of formaldehyde, mercury and other
toxic substances. Compliance issues with workers and the environment are minimized.
Features:
• Ideal & safe transport medium
• Preserves proteins, antigens, carbohydrates, nucleic acids & gross features
• No cross linking of tissues as with formalin-based fixatives
• Minimizes disposal & compliance costs
Catalog # Size
16045-1 1 liter
16045-G 1 gal
00346-1 1 kit
25060-4 4 liters
25058-4 4 liters
25059-1 1 gal
For more information please call (800) 523-2575 or visit: polysciences.com 25