BioSciences - Polysciences, Inc.

Additional Particles
Affinity Binding
Microspheres & Particles
Affinity binding is a straightforward method for immobilizing primary antibodies or biotinylated molecules. Protein A and G and
Fc-specific antibody coatings permit the directed immobilization of primary antibodies, and streptavidin is used extensively for the binding
of biotinylated molecules, such as antibodies, peptides and oligonucleotides.
Biomolecule Coating Strategy Notes
Peptides
Nucleic acids
Proteins (e.g.
antibodies)
Covalent
Streptavidin / biotin
Covalent
Streptavidin / biotin
Covalent
Adsorption
Alternative for BSA as a Blocking Agent
Any innocuous protein may be used to block the effects of non-specific adsorption. In selecting an alternative to BSA, it is suggested that
the size of the active protein and the size of the blocking protein be compared. BSA is highly recommended for IgG coupling. However,
the large size of BSA will obscure the activity of smaller, active proteins. Glycine or small polypeptides may be used as alternatives.
Well dispersed 10µm Polybead ® Microspheres
End-point attachment to preserve the activity of the peptides.
End-point attachment to permit hybridization of probe sequence with target
sequence.
Common proteins are generally large enough that multi-point attachment and
nonspecific orientation do not compromise their activity. However, linkers or
spacers (covalent or SA/B) may be employed to address steric effects or
sub-optimal orientation.
For more information please call (800) 523-2575 or visit: polysciences.com 303