BioSciences - Polysciences, Inc.

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Additional Particles
Washing
Microspheres & Particles
Microspheres sold as instrument standards can often be used as-is, or simply diluted in an appropriate buffer or aqueous solution.
Conversely, microspheres that will be coated or otherwise modified should be washed to remove additives and residuals that could
interfere with the binding reactions or other processes.
Common washing and separation methods for non-magnetic beads include centrifugation, filtration and dialysis. Selection of the “best”
method will depend on scale, required throughput and microsphere characteristics. Centrifugation is often used for small-scale separations
of ≥0.5µm, dialysis for spheres