BioSciences - Polysciences, Inc.

Flow Cytometry
Microspheres & Particles
Catalog # Size
Antibody Binding Standards
Antibody Binding Standards are particles which have ligands covalently linked to their surfaces
to bind specific proteins (e.g. Mouse IgG antibodies) in calibrated quantities.
Tests
Simply Cellular ® anti-Mouse IgG 20 BLI810A-1 1 ml
100 BLI810B-5 5 ml
280 BLI810C-14 14 ml
Simply Cellular ® anti-Human IgG 20 BLI812A-1 1 ml
100 BLI812B-5 5 ml
280 BLI812C-14 14 ml
Simply Cellular ® anti-Rat IgG 20 BLI813A-1 1 ml
100 BLI813B-5 5 ml
280 BLI813C-14 14 ml
QuickCal ® v. 2.3 Data Analysis Program
QuickCal ® constructs a calibration curve associating fluorescence channel values to standardized
fluorescence intensity units. Additionally, the channel value corresponding to the kit’s blank bead
is converted to the appropriate standardized fluorescence intensity unit (MESF or ABC); this will be
the instrument’s detection threshold for the specific reporter and detector. For instructions regarding
QuickCal ® , please see Bangs’ Product Data Sheet 819, QuickCal ® v. 2.3 Data Analysis Program.
Applications
Size Calibration
Size Calibration Standards Kit
The Size Calibration Standards Kit includes five highly uniform unlabeled microbead populations
of different diameters (~4-11µm), which can be used to construct calibration plots for electronic
volume and light scatter instrumentation.
Tests
100 BLI829B-5 5 ml
280
Count Standards
Flow Cytometry Absolute Count Standard
BLI829C-14 14 ml
A2adm
Consists of highly uniform cell-sized microspheres labeled with a full spectrum dye and provided
at a known concentration (~1 x106 ). These beads fluoresce over a broad range of the spectrum,
allowing them to be used as count standards in multiple detectors.
Tests
20 BLI580-10 10 ml
Suspension Arrays
Suspension arrays for the flow cytometer feature populations of microspheres coated with different
ligands for the interrogation of multiple targets within a given sample. Ligand-specific microsphere
populations are encoded so that results for corresponding individual targets may be discerned.
Encoding may be in the form of microsphere size and/or fluorescence signature, as with our
QuantumPlex and QuantumPlex M platforms.
QuantumPlex
The microsphere populations in QuantumPlex five-bead kits are encoded with different intensities
of Starfire Red , and microspheres in our ten-bead kits are distinguished by both fluorescence
intensity and size. Starfire Red is a fluorescent dye with unique characteristics that make it ideal
for multiplexing applications. The dye’s broad excitation band allows it to be excited at a number
of wavelengths, and it emits in the red channel (e.g. PE-Cy 5, APC) with very little carry-over into
lower wavelengths, leaving other detectors available for determination of positive binding events
via common reporters such as FITC and PE.
QuantumPlex kits are available with three different surfaces to accommodate the coating strategy
of choice: carboxyl, streptavidin or anti-Mouse IgG.
For more information please call (800) 523-2575 or visit: polysciences.com 277